De Castro Federica, Vergaro Viviana, Benedetti Michele, Baldassarre Francesca, Del Coco Laura, Dell'Anna Maria Michela, Mastrorilli Piero, Fanizzi Francesco Paolo, Ciccarella Giuseppe
Department of Biological and Environmental Sciences and Technologies (DiSTeBA), University of Salento, via Monteroni, 73100 Lecce, Italy.
Institute of Nanotechnology, CNR NANOTEC, Consiglio Nazionale delle Ricerche, via Monteroni, 73100 Lecce, Italy.
ACS Appl Bio Mater. 2020 Oct 19;3(10):6836-6851. doi: 10.1021/acsabm.0c00766. Epub 2020 Sep 24.
Nanoparticle-based drug delivery systems for cancer therapy offer a great promising opportunity as they specifically target cancer cells, also increasing the bioavailability of anticancer drugs characterized by low water solubility. Platicur, Pt(cur) (NH), is a -diamine-platinum(II) complex linked to curcumin. In this work, an ultrasonication method, coupled with layer by layer technology, allows us to obtain highly aqueous stable Platicur nanocolloids of about 100 nm. The visible light-activated Platicur nanocolloids showed an increased drug release and antitumor activity on HeLa cells, with respect to Platicur nanocolloids in darkness. This occurrence could give very interesting insight into selective activation of the nanodelivered Pt(II) complex and possible side-effect lowering. For the first time, the metabolic effects of Platicur nanocolloid photoactivation, in the HeLa cell line, have been investigated using an NMR-based metabolomics approach coupled with statistical multivariate data analysis. The reported results highlight specific metabolic differences between photoactivated and non-photoactivated Platicur NC-treated HeLa cancer cells.
基于纳米颗粒的癌症治疗药物递送系统提供了一个极具前景的机会，因为它们能够特异性地靶向癌细胞，同时还能提高具有低水溶性的抗癌药物的生物利用度。Platicur，Pt(cur)(NH)，是一种与姜黄素相连的二胺铂(II)配合物。在这项工作中，一种超声处理方法与层层技术相结合，使我们能够获得高度水稳定的、约100纳米的Platicur纳米胶体。与黑暗中的Platicur纳米胶体相比，可见光激活的Platicur纳米胶体在HeLa细胞上显示出更高的药物释放和抗肿瘤活性。这种现象可以为纳米递送的Pt(II)配合物的选择性激活以及可能降低副作用提供非常有趣的见解。首次使用基于核磁共振的代谢组学方法结合统计多变量数据分析，研究了Platicur纳米胶体光激活在HeLa细胞系中的代谢效应。报告的结果突出了光激活和未光激活的Platicur NC处理的HeLa癌细胞之间的特定代谢差异。
