Dong Hui, Cao Linlin, Tan Zhaoling, Liu Qing, Zhou Jin, Zhao Pingping, Wang Ping, Li Yueyun, Ma Wanshan, Dong Yunhui
School of Chemistry and Chemical Engineering, Shandong University of Technology, Zibo 255049, People's Republic of China.
Department of Laboratory Medicine, Shandong Provincial Qianfoshan Hospital, Shandong University, Jinan 250014, People's Republic of China.
ACS Appl Bio Mater. 2020 Jan 21;3(1):377-384. doi: 10.1021/acsabm.9b00863. Epub 2019 Dec 26.
An early diagnosis of cardiovascular disease is of great importance to patients. Herein, a sandwich-type immunosensor for the detection of cardiac troponin I (cTnI) is prepared by using gold nanoparticles (Au NPs) and a composite (CuPtRh CNBs/NH-TiC) of trimetallic hollow CuPtRh cubic nanobox (CNB)-embedded few-layer ultrathin ammoniated MXene (NH-TiC). The embedding of the CuPtRh CNBs into NH-TiC successfully prevents the restacking of NH-TiC and offers more active sites for catalytic HO reduction, which can effectively increase the current signal of the immunosensor. The designed sandwich-type electrochemical immunosensor for detection cTnI has a detection range of 25 fg mL to 100 ng mL, and the detection limit is 8.3 fg mL (S/N = 3). Moreover, the immunosensor also demonstrates high sensitivity, acceptable reproducibility, and good stability. More importantly, the designed system offers a direction for early clinical testing of cardiovascular and cerebrovascular diseases.
心血管疾病的早期诊断对患者至关重要。在此，通过使用金纳米颗粒（Au NPs）和嵌入三金属空心CuPtRh立方纳米盒（CNB）的少层超薄氨化MXene（NH-TiC）复合材料（CuPtRh CNBs/NH-TiC）制备了一种用于检测心肌肌钙蛋白I（cTnI）的夹心型免疫传感器。将CuPtRh CNBs嵌入NH-TiC成功地防止了NH-TiC的重新堆叠，并为催化HO还原提供了更多活性位点，这可以有效地增加免疫传感器的电流信号。所设计的用于检测cTnI的夹心型电化学免疫传感器的检测范围为25 fg mL至100 ng mL，检测限为8.3 fg mL（S/N = 3）。此外，该免疫传感器还具有高灵敏度、可接受的重现性和良好的稳定性。更重要的是，所设计的系统为心脑血管疾病的早期临床检测提供了一个方向。
