Singh Amanpreet, Bains Deepak, Hassen Walid M, Singh Narinder, Dubowski Jan J
Laboratory for Quantum Semiconductors and Photon-Based BioNanotechnology, Interdisciplinary Institute for Technological Innovation (3IT), CNRS UMI-3463, Department of Electrical and Computer Engineering, Université de Sherbrooke, 3000 boul. de l'Université, Sherbrooke, Québec J1K 0A5, Canada.
Department of Chemistry, Indian Institute of Technology Ropar, Punjab 140001, India.
ACS Appl Bio Mater. 2020 Jan 21;3(1):477-485. doi: 10.1021/acsabm.9b00932. Epub 2020 Jan 2.
The spontaneous electron transfer between GaAs and ionic gold through the galvanic displacement reaction results in the formation of gold nanoparticles and a AuGa alloy. We investigated this process for decorating and , aiming at enhanced imaging of these bacteria. The surface of bacteria was modified with gold ions through the electrostatic linkage of ionic liquids with phosphate units of the bacterial cell wall. The modified bacteria were further incubated with an antibody-functionalized GaAs substrate. Due to a large gap in the reduction potential of gold and gallium ions, the induced reaction involving bacteria resulted in a reduction of the gold ions to gold nanoparticles and oxidation of GaAs to GaO and a AuGa alloy. The bacteria covered with a Au/AuGa nanoshell, if excited at 377 nm, show a bright emission at 447 nm originating from Au/AuGa. This approach offers a simple and potentially less expensive method for high-contrast imaging of bacteria in comparison to the conventional methods of staining with different dyes or by conjugating green fluorescent proteins.
通过电偶置换反应，砷化镓与离子态金之间的自发电子转移导致金纳米颗粒和金镓合金的形成。我们研究了这一用于修饰和……的过程，旨在增强对这些细菌的成像。通过离子液体与细菌细胞壁磷酸盐单元的静电连接，用金离子修饰细菌表面。将经修饰的细菌与抗体功能化的砷化镓底物进一步孵育。由于金离子和镓离子还原电位的巨大差异，涉及细菌的诱导反应导致金离子还原为金纳米颗粒，砷化镓氧化为氧化镓和金镓合金。覆盖有金/金镓纳米壳的细菌，若在377nm激发，会在447nm处发出源自金/金镓的明亮发射光。与用不同染料染色或通过共轭绿色荧光蛋白的传统方法相比，这种方法为细菌的高对比度成像提供了一种简单且可能成本较低的方法。
