Gaukås Nikolai Helth, Huynh Quy-Susan, Pratap Anishchal A, Einarsrud Mari-Ann, Grande Tor, Holsinger R M Damian, Glaum Julia
Department of Materials Science and Engineering, NTNU Norwegian University of Science and Technology, Sem Sælands vei 12, Trondheim, Norway.
Laboratory of Molecular Neuroscience and Dementia, Brain and Mind Centre, Faculty of Medicine and Health, The University of Sydney, 94 Mallett St., Camperdown, NSW 2050, Australia.
ACS Appl Bio Mater. 2020 Dec 21;3(12):8714-8721. doi: 10.1021/acsabm.0c01111. Epub 2020 Nov 6.
Lead-free piezoelectric ceramics like KNaNbO (KNN) represent an emerging class of biomaterials for medical technology, as they can be used as components in implantable microelectromechanical systems (MEMS) and bioactive scaffolds for tissue stimulation. Such functional materials can act as working components in future devices, and their addition to current implant designs can greatly improve the biological interaction between host and implant. Despite this, only a few reports have studied the biocompatibility of these materials with living cells. In this work, we investigate the biological response of two different cell lines grown on KNN thin films, and we demonstrate excellent biocompatibility of the KNN films with the cells. Undoped and 0.5 mol % CaTiO-doped KNN thin films with nanometer-sized roughness were deposited on platinized silicon (SiPt) substrates, and cell proliferation, viability, and morphology of human 161BR fibroblast cells and rat Schwann cells grown on the KNN films and SiPt substrates were investigated and compared to glass control samples. The results show that proliferation rates for the cells grown on the KNN thin films were equally high or higher than those on the glass control samples, and no cytotoxic effect from either the films or the substrate was observed. The work demonstrates that KNN thin films on SiPt substrates are very promising candidates for components in implantable medical devices.
像铌酸钾钠（KNN）这样的无铅压电陶瓷是一类新兴的用于医疗技术的生物材料，因为它们可用作可植入微机电系统（MEMS）的组件以及用于组织刺激的生物活性支架。这类功能材料可作为未来设备的工作组件，将它们添加到当前的植入物设计中可极大地改善宿主与植入物之间的生物相互作用。尽管如此，仅有少数报告研究了这些材料与活细胞的生物相容性。在这项工作中，我们研究了在KNN薄膜上生长的两种不同细胞系的生物学反应，并证明了KNN薄膜与细胞具有出色的生物相容性。将具有纳米级粗糙度的未掺杂和0.5 mol％钛酸钙掺杂的KNN薄膜沉积在镀铂硅（SiPt）衬底上，研究了在KNN薄膜和SiPt衬底上生长的人161BR成纤维细胞和大鼠雪旺氏细胞的细胞增殖、活力和形态，并与玻璃对照样品进行了比较。结果表明，在KNN薄膜上生长的细胞的增殖率与在玻璃对照样品上的增殖率相同或更高，并且未观察到薄膜或衬底产生的细胞毒性作用。这项工作表明，SiPt衬底上的KNN薄膜是可植入医疗设备组件的非常有前途的候选材料。
