Faculty of Engineering, Yokohama National University, 79-5 Tokiwadai, Hodogaya-ku, Yokohama, Kanagawa 240-8501, Japan.
Faculty of Engineering, Yokohama National University, 79-5 Tokiwadai, Hodogaya-ku, Yokohama, Kanagawa 240-8501, Japan; Kanagawa Institute of Industrial Science and Technology, 3-25-22 Tonomachi, Kawasaki, Kanagawa 210-0821, Japan.
J Biosci Bioeng. 2022 Mar;133(3):281-290. doi: 10.1016/j.jbiosc.2021.12.003. Epub 2022 Jan 13.
Hair follicle dermal papilla cells (DPCs) are specialized mesenchymal cells that play pivotal roles in hair formation, growth, and cycles, and they are considered as a cell source in hair regenerative medicine. Rodent dermal papilla cells have been shown to induce de novo hair follicle generation in the skin of recipients following transplantation, suggesting that dermal papilla cells can reprogram epidermal microenvironments. However, human DPCs (hDPCs) lose their ability to generate de novo hair follicles under conventional culture methods. We investigated the effects of electrical stimulation (ES) on hDPCs to restore the depressed trichogenic activity. We demonstrated that ES with a polypyrrole (PPy)-modified electrode upregulated trichogenic gene expression in hDPCs in vitro, and the activated cells when transplanted into mice generated double the number of hairs compared to that without the ES. Using specific inhibitors, we revealed that the mechanisms behind the electrical activation are associated with voltage-gated ion channels. Further, ES can be adapted for hDPCs from a patient with androgenic alopecia. Thus, this approach is potentially beneficial in preparing hDPCs for hair regenerative medicine.
毛囊真皮乳头细胞（DPC）是一种特化的间充质细胞，在毛发的形成、生长和周期中起着关键作用，被认为是毛发再生医学中的细胞来源。研究表明，将啮齿动物真皮乳头细胞移植到受体皮肤中后，可诱导新的毛囊生成，这表明真皮乳头细胞可以重编程表皮微环境。然而，在常规培养方法下，人真皮乳头细胞（hDPC）失去了生成新毛囊的能力。我们研究了电刺激（ES）对 hDPC 的影响，以恢复其被抑制的毛发发生活性。我们证明了使用聚吡咯（PPy）修饰电极进行 ES 可上调 hDPC 中毛发发生相关基因的表达，并且激活的细胞在移植到小鼠中后产生的毛发数量是未经 ES 处理的细胞的两倍。通过使用特定的抑制剂，我们揭示了电激活的机制与电压门控离子通道有关。此外，ES 可适用于雄激素性脱发患者的 hDPC。因此，这种方法可能有助于为毛发再生医学制备 hDPC。
