Faculty of Engineering, Yokohama National University, 79-5 Tokiwadai, Hodogaya-ku, Yokohama, Kanagawa, 240-8501, Japan.
Kanagawa Institute of Industrial Science and Technology, 3-2-1 Sakado, Takatsu-ku, Kawasaki, Kanagawa, 213-0012, Japan.
Sci Rep. 2023 Mar 24;13(1):4847. doi: 10.1038/s41598-023-31842-y.
In vitro models of human hair follicle-like tissue could be fundamental tools to better understand hair follicle morphogenesis and hair drug screening. During prenatal development and postnatal cyclic hair regeneration, hair follicle morphogenesis is triggered by reciprocal interactions and the organization of the epithelial and mesenchymal cell populations. Given this mechanism, we developed an approach to induce hair peg-like sprouting in organoid cultures composed of epithelial and mesenchymal cells. Human fetal/adult epithelial and mesenchymal cells were cultured in a medium supplemented with a low concentration of either Matrigel or collagen I. These extracellular matrices significantly enhanced the self-organization capabilities of the epithelial and mesenchymal cells, resulting in spherical aggregation and subsequent hair peg-like sprouting. The length of the hair peg sprouting and associated gene expression significantly increased in the presence of a well-known hair drug, minoxidil. This approach may be beneficial for testing hair growth-promoting drug candidates.
体外人类毛囊样组织模型可以成为更好地理解毛囊形态发生和毛发生长药物筛选的基本工具。在胚胎发育和产后周期性毛发再生过程中，毛囊形态发生是由上皮细胞和间充质细胞的相互作用和组织引起的。鉴于这种机制，我们开发了一种方法来诱导类器官培养物中毛囊样芽的萌发，该类器官培养物由上皮细胞和间充质细胞组成。将人胎儿/成人上皮细胞和间充质细胞在补充有低浓度 Matrigel 或胶原蛋白 I 的培养基中培养。这些细胞外基质显著增强了上皮细胞和间充质细胞的自组织能力，导致球形聚集和随后的毛囊样芽的萌发。在存在已知的毛发生长促进药物米诺地尔的情况下，毛囊样芽的萌发长度和相关基因表达显著增加。这种方法可能有助于测试促进毛发生长的候选药物。
