Cui Jie, Liu Junli, Li Junliang, Cheng Dayou, Dai Cuihong
Harbin Institute of Technology, Harbin, Heilongjiang, China.
College of Life and Environmental Science, Wenzhou University, Wenzhou, Zhejiang, China.
PeerJ. 2022 Jan 5;10:e12719. doi: 10.7717/peerj.12719. eCollection 2022.
In eukaryotes, -methyladenosine (mA) is the most abundant and highly conserved RNA modification. , mA demethylase dynamically regulates the mA level by removing the mA marker where it plays an important role in plant growth, development and response to abiotic stress. The confirmed mA demethylases in include ALKBH9B and ALKBH10B, both belonging to the ALKB family. In this study, BvALKB family members were identified in sugar beet genome-wide database, and their conserved domains, gene structures, chromosomal locations, phylogeny, conserved motifs and expression of genes were analyzed. Almost all BvALKB proteins contained the conserved domain of 2OG-Fe II-Oxy. Phylogenetic analysis suggested that the ten proteins were clustered into five groups, each of which had similar motifs and gene structures. Three mA demethylase-homologous proteins (BvALKBH6B, BvALKBH8B and BvALKBH10B) were of particular interest in our study. Expression profile analysis showed that almost all genes were up-regulated or down-regulated to varying degrees under salt stress. More specifically, homologous to was significantly up-regulated, suggesting that the transcriptional activity of this gene is responsive to salt stress. This study provides a theoretical basis for further screening of mA demethylase in sugar beet, and also lays a foundation for studying the role of ALKB family proteins in growth, development and response to salinity stress.
在真核生物中，N6-甲基腺苷（m6A）是最丰富且高度保守的RNA修饰。m6A去甲基化酶通过去除m6A标记来动态调节m6A水平，其在植物生长、发育及对非生物胁迫的响应中发挥重要作用。在植物中已确认的m6A去甲基化酶包括ALKBH9B和ALKBH10B，二者均属于ALKB家族。本研究在甜菜全基因组数据库中鉴定了BvALKB家族成员，并分析了它们的保守结构域、基因结构、染色体定位、系统发育、保守基序及基因表达。几乎所有BvALKB蛋白都含有2OG-Fe II-Oxy保守结构域。系统发育分析表明，这10个蛋白被聚类为5组，每组具有相似的基序和基因结构。本研究特别关注3个m6A去甲基化酶同源蛋白（BvALKBH6B、BvALKBH8B和BvALKBH10B）。表达谱分析表明，几乎所有基因在盐胁迫下均有不同程度的上调或下调。更具体地说，与AtALKBH10B同源的基因显著上调，表明该基因的转录活性对盐胁迫有响应。本研究为进一步筛选甜菜中的m6A去甲基化酶提供了理论依据，也为研究ALKB家族蛋白在生长、发育及对盐胁迫响应中的作用奠定了基础。
