JEOL USA, Inc., Peabody, Massachusetts, USA.
Rapid Commun Mass Spectrom. 2022 Apr 30;36(8):e9258. doi: 10.1002/rcm.9258.
A proof of concept showing GC-MS/MS analysis time for pesticides can be dramatically reduced while maintaining a similar separation efficiency by combining a low-pressure gas chromatography (LPGC) column with the enhanced selected reaction monitoring (SRM) switching speed of the short collision cell of a JEOL JMS-TQ4000GC.
Triple-quadrupole tandem mass spectrometry (standard EI + at 70 eV) was used to measure pesticides eluting from a low-pressure gas chromatograph capillary column. Three transitions for each of 244 pesticide compounds were measured within an 11-min analysis time, and the data were checked to confirm the method's reproducibility and ability to distinguish all three transitions for each pesticide.
All three transitions for all 244 pesticides were detected in the standard mixture at 1X concentration within the 11-min analysis time. Relative standard deviation (RSD) of peak areas was less than 15% for 242 pesticides, and I/Q RSDs were less than 10% for 242 compounds. Retention time RSD over 15 replicates was less than 0.1%.
Results show that analysis time can be markedly decreased using an LPGC column, and that the ability of the short collision cell to distinguish a large number of coeluting peaks makes the two technologies a natural pairing. The effective measurement of pesticides within a short time could benefit any scientists doing pesticide analysis work.
通过将低压气相色谱 (LPGC) 柱与 JEOL JMS-TQ4000GC 短碰撞池的增强选择反应监测 (SRM) 切换速度相结合，可在保持相似分离效率的同时，显著缩短农药的 GC-MS/MS 分析时间。
采用三重四极杆串联质谱（标准 EI+在 70 eV）测量从低压气相色谱毛细管柱洗脱的农药。在 11 分钟的分析时间内测量了 244 种农药化合物中的每一种的三个跃迁，并且检查了数据以确认该方法的重现性和区分每种农药的所有三个跃迁的能力。
在 11 分钟的分析时间内，标准混合物中的所有 244 种农药的所有三个跃迁都在 1X 浓度下被检测到。242 种农药的峰面积相对标准偏差 (RSD) 小于 15%，242 种化合物的 I/Q RSD 小于 10%。超过 15 个重复的保留时间 RSD 小于 0.1%。
结果表明，使用 LPGC 柱可以显著缩短分析时间，并且短碰撞池区分大量共洗脱峰的能力使这两种技术成为自然配对。在短时间内有效测量农药可能会使任何从事农药分析工作的科学家受益。
