Pandya Nirali, Kumar Amit
Department of Biosciences and Biomedical Engineering, Indian Institute of Technology Indore, Indore, Madhya Pradesh, India.
J Biomol Struct Dyn. 2023 Mar;41(5):1859-1878. doi: 10.1080/07391102.2021.2025430. Epub 2022 Jan 18.
The development of T cell and B cell that able provide long-term immune response against the schistosomiasisis to the people belongs to the epidemic area. Heat Shock Proteins (HSPs) are up-regulated in schistosomes as their environment changes owing to the developmental cycle, assisting the parasite in living with the adverse circumstances related with its life cycle. Schistosomiasis is still a severe health problem in the people of many countries in worldwide. In this work, to develop a chimeric antigen, we used an advanced and powerful immunoinformatics technique that targeted () Heat shock protein (HSPs). Antigenicity, immunogenicity, allergenicity, and physicochemical characteristics were all assessed for the developed subunit vaccine. The 3D structure of the vaccine was constructed and the stability of the vaccine construct was increased by using disulphide engineering. The protein-protein docking and simulation were performed between the vaccine construct and Toll-like receptor-4. The antigenicity probability value obtained for the vaccine construct was 0.93, which indicates that vaccine is non-allergenic and safe for human consumption. Communicated by Ramaswamy H. Sarma.
能够为疫区人群提供针对血吸虫病的长期免疫反应的T细胞和B细胞的发育。由于发育周期，血吸虫中的热休克蛋白（HSPs）随着其环境变化而上调，帮助寄生虫在与其生命周期相关的不利环境中生存。血吸虫病在世界许多国家的人群中仍然是一个严重的健康问题。在这项工作中，为了开发一种嵌合抗原，我们使用了先进且强大的免疫信息学技术，该技术靶向（）热休克蛋白（HSPs）。对所开发的亚单位疫苗的抗原性、免疫原性、致敏性和理化特性进行了评估。构建了疫苗的三维结构，并通过二硫键工程提高了疫苗构建体的稳定性。在疫苗构建体和Toll样受体-4之间进行了蛋白质-蛋白质对接和模拟。疫苗构建体获得的抗原性概率值为0.93，这表明该疫苗无致敏性，对人类消费安全。由拉马斯瓦米·H·萨尔马传达。
