Onile Olugbenga S, Fadahunsi Adeyinka I, Adekunle Ameerah A, Oyeyemi Bolaji F, Anumudu Chiaka I
Biotechnology Programme, Department of Biological Sciences, Elizade University, Ilara-Mokin, Ondo State, Nigeria.
Molecular Biology Group, Department Science Technology, The Federal Polytechnic, Ado-Ekiti, Ado-Ekiti, Ekiti State, Nigeria.
PeerJ. 2020 Oct 2;8:e8795. doi: 10.7717/peerj.8795. eCollection 2020.
Discovery of T and B memory cells capable of eliciting long-term immunity against schistosomiasisis is important for people in endemic areas. Changes in schistosomes environment due to developmental cycle, induces up-regulation of Heat Shock Proteins (HSPs) which assist the parasite in coping with the hostile conditions associated with its life cycle. This study therefore focused on exploring the role of HSPs in urogenital schistosomiasis to develop new multi-epitope subunit vaccine against the disease using immunoinformatic approaches. The designed subunit vaccine was subjected to in silico antigenicity, immunogenicity, allergenicity and physicochemical parameters analysis. A 3D structure of the vaccine construct was predicted, followed by disulphide engineering for stability, codon adaptation and in silico cloning for proper expression and molecular protein-protein docking of vaccine construct in the vector against toll-like receptor 4 receptor, respectively. Consequently, a 493 amino acid multi-epitope vaccine construct of antigenicity probability of 0.91 was designed. This was predicted to be stable, non-allergenic in nature and safe for human use.
发现能够引发针对血吸虫病的长期免疫力的T细胞和B记忆细胞，对流行地区的人们来说非常重要。由于发育周期导致的血吸虫环境变化，会诱导热休克蛋白（HSPs）上调，这些热休克蛋白有助于寄生虫应对与其生命周期相关的恶劣条件。因此，本研究着重探索热休克蛋白在泌尿生殖系统血吸虫病中的作用，利用免疫信息学方法开发针对该疾病的新型多表位亚单位疫苗。对设计的亚单位疫苗进行了计算机模拟的抗原性、免疫原性、致敏性和理化参数分析。预测了疫苗构建体的三维结构，随后进行二硫键工程以提高稳定性、密码子优化以及计算机模拟克隆以实现正确表达，并分别对疫苗构建体与Toll样受体4受体在载体中的分子蛋白-蛋白对接进行了研究。最终，设计出了一种抗原性概率为0.91的493个氨基酸的多表位疫苗构建体。预计该构建体性质稳定、无致敏性且对人类使用安全。
