视觉皮层在实验性高眼压症雪貂模型中的损伤。

Visual cortex damage in a ferret model of ocular hypertension.

机构信息

Department of Ophthalmology, University of Tokyo School of Medicine, 7-3-1 Hongo Bunkyoku, Tokyo, 113-8655, Japan.

Department of Medical Neuroscience, Graduate School of Medicine, Kanazawa University, 13-1 Takaramachi, Kanazawashi, Kanazawa, Japan.

出版信息

Jpn J Ophthalmol. 2022 Mar;66(2):205-212. doi: 10.1007/s10384-022-00901-8. Epub 2022 Jan 19.

DOI:10.1007/s10384-022-00901-8

PMID:35044565

Abstract

PURPOSE

We aimed to analyze the changes in the visual cortex of a ferret model of ocular hypertension (OH) using cytochrome oxidase (CO) staining.

STUDY DESIGN

Experimental.

METHODS

OH was induced in 9 ferrets by means of injection of cultured conjunctival cells into the anterior chamber of the right eye. Three ferrets were used as the controls. CO staining was performed to assess the metabolic intensity at the II-III and IVC layers of the visual cortex.

RESULTS

The intensities of CO staining in the right and left II-III layers of the primary visual cortex (V1) in the OH ferrets were 39.8 ± 10.3 and 41.9 ± 9.2 arbitrary units, respectively. In the control ferrets, the intensity was 88.1 ± 8.1 arbitrary units. The intensity of CO staining of the II-III layers obtained from the OH eyes was significantly lower than that from the control eyes (unpaired t test, P < .01). The intensities of CO staining in the right and left IVC layers of V1 in the OH ferrets were 60.3 ± 12.8 and 60.0 ± 13.5 arbitrary units, respectively. In the control ferrets, the intensity was 111.4 ± 9.6 arbitrary units. The CO staining intensity of the IVC layer obtained from the OH eyes was significantly lower than that from the control eyes (unpaired t test, P < .01).

CONCLUSION

The CO staining intensity was reduced in the visual cortex from OH eyes. This study revealed that OH causes metabolic change in the visual cortex.

摘要

目的

我们旨在通过细胞色素氧化酶（CO）染色分析实验性高眼压（OH）模型中视皮层的变化。

设计

实验。

方法

通过将培养的结膜细胞注射到右眼前房，在 9 只雪貂中诱导 OH。3 只雪貂作为对照。进行 CO 染色以评估视皮层 II-III 和 IVC 层的代谢强度。

结果

OH 雪貂右眼和左眼初级视皮层（V1）的 II-III 层 CO 染色强度分别为 39.8±10.3 和 41.9±9.2 任意单位。在对照雪貂中，强度为 88.1±8.1 任意单位。OH 眼获得的 II-III 层 CO 染色强度明显低于对照眼（配对 t 检验，P<.01）。V1 的右和左 IVC 层 CO 染色强度分别为 60.3±12.8 和 60.0±13.5 任意单位。在对照雪貂中，强度为 111.4±9.6 任意单位。OH 眼获得的 IVC 层 CO 染色强度明显低于对照眼（配对 t 检验，P<.01）。