Tigges M, Hendrickson A E, Tigges J
J Comp Neurol. 1984 Jul 20;227(1):1-13. doi: 10.1002/cne.902270103.
The effects of long-term monocular deprivation on the geniculostriate system in squirrel monkeys were studied with neuroanatomical methods. Four neonates were visually deprived by monocular eyelid suture during their first 10 days of life and survived from 9 to 40 months. In the lateral geniculate nucleus (LGN), deprivation resulted in severe cell size changes. Neurons in the deprived laminae were smaller compared to those in the undeprived laminae. Deprivation left the reciprocal connections between LGN and striate cortex intact: After horseradish peroxidase (HRP) injections into striate cortex, retrogradely transported enzyme labeled a wedge of neurons in deprived and undeprived LGN laminae; anterogradely transported HRP filled preterminal and terminal axons in this wedge. Following 3H-proline injections into the deprived eye for transneuronal transport, autoradiography showed in the ipsilateral striate cortex a silver grain distribution over most of layer IVc similar to that in normal squirrel monkeys, except for a small strip in the anterior calcarine fissure. Here, a few, irregularly spaced "patches" of higher grain density occurred deep in layer IVc. Layer IVc of contralateral area 17 was also uniformly labeled over most of its extent, except for a very few and inconspicuous accumulations of slightly increased silver grains. After visual stimulation of the deprived eye, the 14C-2-deoxyglucose method showed in the contralateral striate cortex some alternating "patches" of higher uptake superimposed on the heavy labeling in layer IVc. Layer IVc in the ipsilateral cortex was more uniformly labeled. Regularly spaced arrays of labeled "puffs" in layers II/III were present in both hemispheres. Cytochrome oxidase staining showed no change in the distribution pattern of the enzyme in the deprived monkeys from the basic pattern of normal adults. No changes in cell sizes were found in layer IVc in cresyl-violet-acetate-stained sections. These results lead to the conclusion that in area 17 of squirrel monkeys there is no distinct segregation of inputs from the two eyes into anatomically discrete ocular dominance columns and they support the view of a predominantly binocular organization of area 17.
采用神经解剖学方法研究了长期单眼剥夺对松鼠猴膝状纹状体系统的影响。4只新生猴在出生后的头10天通过单眼眼睑缝合进行视觉剥夺，并存活了9至40个月。在外侧膝状核（LGN）中，剥夺导致严重的细胞大小变化。与未剥夺层中的神经元相比，剥夺层中的神经元较小。剥夺并未破坏LGN与纹状皮质之间的相互连接：将辣根过氧化物酶（HRP）注入纹状皮质后，逆行运输的酶标记了剥夺和未剥夺LGN层中的楔形神经元；顺行运输的HRP填充了该楔形区域中的终末前和终末轴突。在将³H-脯氨酸注入剥夺眼进行跨神经元运输后，放射自显影显示，在同侧纹状皮质中，除了距状裂前部的一小条区域外，IVc层大部分区域的银粒分布与正常松鼠猴相似。在此处，IVc层深处出现了一些间隔不规则、密度较高的“斑块”。对侧17区的IVc层在其大部分范围内也均匀标记，除了极少数不明显的、银粒略有增加的聚集区。对剥夺眼进行视觉刺激后，¹⁴C-2-脱氧葡萄糖法显示，在对侧纹状皮质中，IVc层的大量标记之上叠加了一些交替出现的、摄取较高的“斑块”。同侧皮质的IVc层标记更为均匀。在两个半球的II/III层中均存在规则间隔的标记“膨体”阵列。细胞色素氧化酶染色显示，剥夺猴中该酶的分布模式与正常成年猴的基本模式相比没有变化。在醋酸甲酚紫染色切片的IVc层中未发现细胞大小的变化。这些结果得出结论，在松鼠猴的17区，来自双眼的输入并没有明显分离成解剖学上离散的眼优势柱，它们支持了17区主要是双眼组织的观点。
