Gupta Bhumika, Sharma Garima, Sharma Pratibha, Sandhu Simarjot Kaur, Kaur Indu Pal
University Institute of Pharmaceutical Sciences, Panjab University, Chandigarh 160014, India.
Gels. 2022 Jan 13;8(1):58. doi: 10.3390/gels8010058.
Hydrogels, an advanced interactive system, is finding use as wound dressings, however, they exhibit restricted mechanical properties, macroscopic nature, and may not manage high exudate wounds or incorporate lipophilic actives. In this study, we developed a self-gelling solid lipid nanoparticle (SLNs) dressing to incorporate simvastatin (SIM), a lipophilic, potential wound-healing agent, clinically limited due to poor solubility (0.03 mg/mL) and absorption. The study explores unconventional and novel application of SIM. The idea was to incorporate a significant amount of SIM in a soluble form and release it slowly over a prolonged time. Further, a suitable polymeric surfactant was selected that assigned a self-gelling property to SLNs (SLN-hydrogel) so as to be used as a novel wound dressing. SLNs assign porosity, elasticity, and occlusivity to the dressing to keep the wound area moist. It will also provide better tolerance and sensory properties to the hydrogel. SIM loaded SLN-hydrogel was prepared employing an industry amenable high-pressure homogenization technique. The unique hydrogel dressing was characterized for particle size, zeta potential, Fourier transform infra-red spectroscopy, powder X-ray diffraction, differential scanning calorimetry, rheology, and texture. Significant loading of SIM (10% /) was achieved in spherical nanoparticule hydrogel (0.3 nm (nanoparticles) to 2 µm (gelled-matrix)) that exhibited good spreadability and mechanical properties and slow release up to 72 h. SLN-hydrogel was safe as per the organization for economic co-operation and development (OECD-404) guidelines, with no signs of irritation. Complete healing of excision wound observed in rats within 11 days was 10 times better than marketed povidone-iodine product. The presented work is novel both in terms of classifying a per se SLN-hydrogel and employing SIM. Further, it was established to be a safe, effective, and industry amenable invention.
水凝胶作为一种先进的交互式系统，正被用作伤口敷料，然而，它们表现出有限的机械性能、宏观性质，可能无法处理高渗伤口或纳入亲脂性活性成分。在本研究中，我们开发了一种自凝胶化固体脂质纳米颗粒（SLN）敷料，以纳入辛伐他汀（SIM），一种亲脂性、潜在的伤口愈合剂，由于其溶解度低（0.03 mg/mL）和吸收性差，在临床上受到限制。该研究探索了SIM的非常规和新颖应用。其理念是以可溶形式纳入大量SIM，并在较长时间内缓慢释放。此外，选择了一种合适的聚合物表面活性剂，赋予SLN（SLN-水凝胶）自凝胶化特性，以便用作新型伤口敷料。SLN赋予敷料孔隙率、弹性和封闭性，以保持伤口区域湿润。它还将为水凝胶提供更好的耐受性和感官特性。采用适合工业生产的高压均质技术制备了载有SIM的SLN-水凝胶。对这种独特的水凝胶敷料进行了粒径、zeta电位、傅里叶变换红外光谱、粉末X射线衍射、差示扫描量热法、流变学和质地等表征。在球形纳米颗粒水凝胶（0.3纳米（纳米颗粒）至2微米（凝胶基质））中实现了SIM的显著负载（10%/），该水凝胶具有良好的铺展性和机械性能，并能缓慢释放长达72小时。根据经济合作与发展组织（OECD-404）指南，SLN-水凝胶是安全的，没有刺激迹象。在大鼠中观察到，切除伤口在11天内完全愈合，比市售的聚维酮碘产品好10倍。就将SLN-水凝胶本身进行分类以及使用SIM而言，目前的工作都是新颖的。此外，它被证明是一项安全、有效且适合工业生产的发明。
