Zhang Xin, Yang Fu, Ma Hongyuan, Li Jingpeng
Northeast Institute of Geography and Agroecology, Chinese Academy of Sciences, Changchun 130102, China.
University of Chinese Academy of Sciences, Beijing 100049, China.
Biology (Basel). 2022 Jan 13;11(1):126. doi: 10.3390/biology11010126.
Soil salinity is a widespread and important abiotic factor impeding rice production by adversely affecting seed germination, seedling growth, and plant productivity. In this study, the rice cultivar TH899 was treated with 200 Gy of heavy-ion beam irradiation, and 89 mutant lines with stable phenotypes were selected using the pedigree method based on continuous assessment over six years. The seed germination performance of these mutants was tested under different saline-alkaline concentrations. Five highly tolerant lines were further evaluated in a series of experiments at the seedling stage and in the field. During the seedling stage, the reduction of seedling length, root length, fresh weight, and dry weight were dramatically lower in these five mutants than those in TH899 under saline-alkali stress. The K/Na ratio was higher in these five mutants than in TH899. In the field experiment, the grain yield of mutant lines was higher than that of TH899. In addition, the grain yield of mutant line M89 was higher than that of the local cultivar in actual production. These mutant lines are expected to increase grain yield in soda saline-alkaline regions in northeast China.
土壤盐碱化是一种广泛且重要的非生物胁迫因素，通过对种子萌发、幼苗生长和植株生产力产生不利影响，阻碍水稻生产。在本研究中，水稻品种TH899接受了200 Gy的重离子束辐照，并基于连续六年的评估，采用系谱法筛选出89个表型稳定的突变系。对这些突变体在不同盐碱浓度下的种子萌发性能进行了测试。进一步在苗期和田间的一系列实验中对五个高度耐盐碱系进行了评估。在苗期，在盐碱胁迫下，这五个突变体的苗长、根长、鲜重和干重的降低幅度显著低于TH899。这五个突变体的K/Na比值高于TH899。在田间试验中，突变系的籽粒产量高于TH899。此外，在实际生产中，突变系M89的籽粒产量高于当地品种。这些突变系有望提高中国东北苏打盐碱地地区的粮食产量。
