College of Landscape Architecture, Sichuan Agricultural University, Chengdu 611130, China.
Department of Plant Sciences, University of California Davis, Davis, CA 95616, USA.
Biomolecules. 2021 Feb 22;11(2):327. doi: 10.3390/biom11020327.
The resurrection plants can survive long term severe drought and desiccation conditions and soon recover after rewatering. However, few genes related to such excellent drought tolerance and underlying molecular mechanism have been excavated. WRKY transcription factors play critical roles in biotic and abiotic stress signaling, in which WRKY70 functions as a positive regulator in biotic stress response but a negative regulator in abiotic stress signaling in and some other plant species. In the present study, the functions of a dehydration-induced of participating was investigated in the model plant . Our results indicated that MfWRKY70 was localized in the nucleus and could significantly increase tolerance to drought, osmotic, and salinity stresses by promoting root growth and water retention, as well as enhancing the antioxidant enzyme system and maintaining reactive oxygen species (ROS) homeostasis and membrane-lipid stability under stressful conditions. Moreover, the expression of stress-associated genes (, and ) was positively regulated in the overexpression of We proposed that MfWRKY70 may function as a positive regulator for abiotic stress responses and can be considered as a potential gene for improvement of drought and salinity tolerance in plants.
resurrection plants 可以在长期严重干旱和干燥条件下存活，并在重新浇水后迅速恢复。然而，很少有与这种出色的耐旱性相关的基因被挖掘出来，其潜在的分子机制也是如此。WRKY 转录因子在生物和非生物胁迫信号中起着关键作用，其中 WRKY70 在生物胁迫反应中作为正调节剂，但在 和其他一些植物物种的非生物胁迫信号中作为负调节剂。在本研究中，研究了参与模型植物 脱水诱导 的 的功能。我们的结果表明，MfWRKY70 定位于细胞核，通过促进根的生长和保持水分，增强抗氧化酶系统，以及在胁迫条件下维持活性氧（ROS）平衡和膜脂稳定性，可显著提高对干旱、渗透和盐胁迫的耐受性。此外，胁迫相关基因（ 、 和 ）的表达在 过表达时被正向调控。我们提出，MfWRKY70 可能作为非生物胁迫反应的正调节剂发挥作用，并可被视为提高植物耐旱性和耐盐性的潜在基因。
