Morrison W B, Wilsman N J, Fox L E, Farnum C E
Department of Veterinary Clinical Sciences, Iowa State University, Ames.
J Vet Intern Med. 1987 Apr-Jun;1(2):67-74. doi: 10.1111/j.1939-1676.1987.tb01989.x.
Electron microscopy was used to diagnose primary ciliary dyskinesia in a litter of English pointer dogs and in a golden retriever dog. A technique of membrane solubilization, fixation, and negative staining with glutaraldehyde tannic acid identified abnormally constructed central and B microtubules in respiratory cilia from dogs with primary ciliary dyskinesia. Shortened outer dynein arms commonly associated with primary ciliary dyskinesia actually represents the absence of a specific subset of the three most peripheral components of the whole outer dynein arm structure.
电子显微镜被用于诊断一窝英国指示犬和一只金毛猎犬的原发性纤毛运动障碍。一种通过戊二醛单宁酸进行膜溶解、固定和负染色的技术，在患有原发性纤毛运动障碍的犬类的呼吸道纤毛中，鉴定出结构异常的中央微管和B微管。通常与原发性纤毛运动障碍相关的缩短的外动力蛋白臂，实际上代表整个外动力蛋白臂结构的三个最外围组件中一个特定子集的缺失。
