Key Laboratory of Beijing for Identification and Safety Evaluation of Chinese Medicine, Institute of Chinese Materia Medica, China Academy of Chinese Medical Sciences, No.16, Nanxiaojie Dongzhimennei, Beijing 100700, China.
Amyway (China) Botanical R&D Centre, Wuxi 214115, China.
Food Chem. 2022 Jun 15;379:132124. doi: 10.1016/j.foodchem.2022.132124. Epub 2022 Jan 10.
Untargeted metabolomics was performed to study the profiles of 101 chemicals in lotus seeds using ultrahigh-performance liquid chromatography-photodiode array detection-high-resolution tandem mass spectrometry. Among them, 16 dimeric, 18 trimeric, and 4 tetrameric proanthocyanidins were theoretically identified based on the degree of polymerization, and the number of linkages and the presence of two dihydroflavonols and three glycosylated alkaloids were determined for the first time. The proanthocyanidin, flavonoid, amino acid, and total compound contents were quantified, revealing decreases in their levels during maturation as well as a polymerization process formation of polymers from monomers during seed maturation. Interestingly, glycosylated alkaloids were only detected in seed cotyledons being highest at green-brown stage, whereas proanthocyanidins were present at a concentration of 8,226.19 ± 249.96 μg/g (dry weight) in green-brown stage of seed coats. Our findings may provide insights into the utilization of lotus seeds as a functional food.
采用超高效液相色谱-光电二极管阵列检测-高分辨串联质谱法对莲子中的 101 种化学物质进行非靶向代谢组学研究。其中，根据聚合度、连接数和二氢黄酮醇及三糖苷生物碱的存在，理论上鉴定了 16 种二聚体、18 种三聚体和 4 种四聚体原花青素。定量测定了原花青素、类黄酮、氨基酸和总化合物含量，结果表明在成熟过程中以及在种子成熟过程中单体聚合形成聚合物的过程中，其含量降低。有趣的是，仅在种皮的子叶中检测到糖基化生物碱，在绿褐色阶段含量最高，而原花青素在种皮的绿褐色阶段的浓度为 8226.19±249.96μg/g（干重）。我们的研究结果可能为将莲子作为功能性食品的利用提供参考。
