Department of Food Science, Niigata University of Pharmacy and Applied Life Sciences, Akiha-ku, Niigata, Japan.
J Agric Food Chem. 2011 Apr 27;59(8):3738-46. doi: 10.1021/jf104976n. Epub 2011 Mar 10.
Proanthocyanidins and other polyphenols in the seeds and juice of boysenberry were quantitatively analyzed. Polyphenolic extracts were prepared from the waste seeds and commercial juice by chromatographic fractionation. Compositional analysis revealed that both extracts contained six polyphenolic classes: flavanol monomers, proanthocyanidins, anthocyanins, ellagic acid, ellagitannins, and flavonol glycosides. Ellagitannins were the most abundant polyphenols in both extracts. Proanthocyanidins were present as short oligomers consisting of dimeric and trimeric procyanidins and propelargonidins, with the most abundant component being procyanidin B4 in both extracts. Quantification by high-performance liquid chromatography-mass spectrometry (HPLC-MS) revealed that the seeds contained a 72-fold higher amount of proanthocyanidins than the juice. These results indicate that boysenberry fruits contain short oligomeric proanthocyanidins along with flavanol monomers and the seeds represent a good source of short oligomeric proanthocyanidins.
对黑莓种子和果汁中的原花青素和其他多酚类物质进行了定量分析。通过色谱分离从废料种子和商业果汁中制备了多酚提取物。成分分析表明，两种提取物均含有六种多酚类物质：黄烷醇单体、原花青素、花青素、鞣花酸、鞣花单宁和黄酮醇糖苷。鞣花单宁是两种提取物中含量最丰富的多酚类物质。原花青素以二聚体和三聚体原花青素和丙二酰基原花青素组成的短低聚物形式存在，两种提取物中最丰富的成分均为原花青素 B4。高效液相色谱-质谱联用（HPLC-MS）定量分析表明，种子中原花青素的含量是果汁的 72 倍。这些结果表明，黑莓果实中含有短低聚物原花青素以及黄烷醇单体，种子是短低聚物原花青素的良好来源。
