Department of Parasitology and Mycology, School of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran.
Department of Parasitology and Mycology, School of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran.
Infect Genet Evol. 2022 Mar;98:105222. doi: 10.1016/j.meegid.2022.105222. Epub 2022 Jan 20.
Leishmania RNA virus (LRV) is a double-strand RNA virus that was first detected in members of the Leishmania viannia in the New World. The present study aimed to investigate the presence of LRV in the Leishmania species isolated from cutaneous leishmaniasis (CL) patients and rodents as reservoirs in Isfahan province an old zoonotic CL focus, center of Iran. Totally, 85 samples were collected from CL patients (n = 80) and rodent reservoirs (n = 5) from different regions of Isfahan province. Species identification was determined using the PCR-RFLP method. Viral dsRNA was extracted and for observation of 5.3 kb dsRNA on an agarose gel. The presence of LRV was surveyed using the Semi-nested PCR method. For phylogenetic analyzes, 6 samples of 13 isolates were sequenced and a phylogenetic tree was drawn by MEGA7 version 7.0.26. Of 80 Leishmania isolates recovered from the patients with CL, 79 and only one were identified as L. major and L. tropica, respectively. Also, the PCR assays detected four L. major and one L. turanica in five assessed Rhombomys opimus as the rodent reservoirs. LRV was detected only in Leishmania species isolated from 13 species of 85 (15.3%) CL including (L. major, n = 12) and (L. tropica, n = 1). Phylogenetic analysis showed that they were belonged to LRV2 and had the highest similarity with Iranian reference LRV2 in GenBank. Our results showed that the LRV2 was present in cutaneous Leishmania species in Isfahan province is the most historical and touristic province of Iran. In the study LRV was not reported from rodent reservoirs, it may be due to the small sample size. Phylogenetic analysis of current sequences demonstrated that these isolates belong to the registered LRV2 of the Old World.
利什曼原虫 RNA 病毒（LRV）是一种双链 RNA 病毒，最初在新世界的利什曼原虫属成员中被发现。本研究旨在调查在伊朗中部历史悠久的人畜共患皮肤利什曼病（CL）旧疫区伊斯法罕省分离的皮肤利什曼原虫（CL）患者和啮齿动物（作为储存库）中是否存在 LRV。共从伊斯法罕省不同地区的 CL 患者（n=80）和啮齿动物储存库（n=5）中采集了 85 个样本。采用 PCR-RFLP 法确定种属鉴定。提取病毒 dsRNA，在琼脂糖凝胶上观察 5.3kb dsRNA。采用半巢式 PCR 法检测 LRV 的存在。为了进行系统发育分析，对 13 个分离株中的 6 个样本进行测序，并通过 MEGA7 版本 7.0.26 绘制系统发育树。从 CL 患者中分离出的 80 株利什曼原虫中，79 株和 1 株分别鉴定为 L. major 和 L. tropica。此外，PCR 检测在 5 只评估的 R. opimus 啮齿动物储存库中检测到 4 株 L. major 和 1 株 L. turanica。仅在从 85 株 CL 中分离的 13 株利什曼原虫中检测到 LRV（包括 L. major，n=12 和 L. tropica，n=1）。系统发育分析表明，它们属于 LRV2，与 GenBank 中的伊朗参考 LRV2 具有最高的相似性。我们的结果表明，在伊朗历史最悠久、最具旅游特色的伊斯法罕省，皮肤利什曼原虫种属中存在 LRV2。在这项研究中，未从啮齿动物储存库中报告 LRV，这可能是由于样本量小。当前序列的系统发育分析表明，这些分离株属于已注册的旧世界 LRV2。
