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基于铂配位钛基卟啉金属有机框架构建用于凝血酶检测的电化学适体传感器

Construction of Electrochemical Aptamer Sensor Based on Pt-Coordinated Titanium-Based Porphyrin MOF for Thrombin Detection.

作者信息

Jiang Jiazi, Cai Quan, Deng Minghan

机构信息

Department of Emergency, The First Hospital of China Medical University, Shenyang, China.

Department of Laboratory, The First Hospital of China Medical University, Shenyang, China.

出版信息

Front Chem. 2022 Jan 7;9:812983. doi: 10.3389/fchem.2021.812983. eCollection 2021.

DOI:10.3389/fchem.2021.812983
PMID:35071191
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC8776986/
Abstract

In this work, a Pt-coordinated titanium-based porphyrin metal organic framework (Ti-MOF-Pt) was prepared by embedding single-atom Pt through strong interactions between the four pyrrole nitrogen atoms in the rigid backbone of the porphyrin. The synthesized Ti-MOF-Pt was characterized by TEM, XRD, FTIR and BET. Then, the Ti-MOF-Pt has been used for glassy carbon electrode surface modification and consequently used for construction of a thrombin aptamer sensor. The high surface area provides by MOF and excellent electrochemical property provided by Pt enhance the sensing performance. After optimization of amount of aptamer, hybridization time and specific reaction time, the fabricated aptamer sensor exhibited a linear relationship with the logarithm of the thrombin concentration in the range of 4 pM to 0.2 μM. The detection limit can be calculated as 1.3 pM.

摘要

在本工作中，通过卟啉刚性骨架中四个吡咯氮原子之间的强相互作用嵌入单原子铂，制备了一种铂配位的钛基金属有机骨架（Ti-MOF-Pt）。通过透射电子显微镜（TEM）、X射线衍射（XRD）、傅里叶变换红外光谱（FTIR）和比表面积分析仪（BET）对合成的Ti-MOF-Pt进行了表征。然后，将Ti-MOF-Pt用于玻碳电极表面修饰，并进而用于构建凝血酶适配体传感器。金属有机骨架提供的高比表面积和铂提供的优异电化学性能增强了传感性能。在优化适配体用量、杂交时间和特异性反应时间后，所制备的适配体传感器与凝血酶浓度的对数在4 pM至0.2 μM范围内呈线性关系。检测限可计算为1.3 pM。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5cd7/8776986/b39a1a2da2e4/fchem-09-812983-g010.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5cd7/8776986/135aaec71723/fchem-09-812983-g001.jpg
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https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5cd7/8776986/72dd7ebea330/fchem-09-812983-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5cd7/8776986/23c9bd6fed9f/fchem-09-812983-g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5cd7/8776986/f4c17e4ac8e7/fchem-09-812983-g008.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5cd7/8776986/e618ae7738e8/fchem-09-812983-g009.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5cd7/8776986/b39a1a2da2e4/fchem-09-812983-g010.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5cd7/8776986/135aaec71723/fchem-09-812983-g001.jpg
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https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5cd7/8776986/f8a7a1a4caf7/fchem-09-812983-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5cd7/8776986/72dd7ebea330/fchem-09-812983-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5cd7/8776986/23c9bd6fed9f/fchem-09-812983-g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5cd7/8776986/f4c17e4ac8e7/fchem-09-812983-g008.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5cd7/8776986/e618ae7738e8/fchem-09-812983-g009.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5cd7/8776986/b39a1a2da2e4/fchem-09-812983-g010.jpg

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