Liu Kun, Luo Xin, Lv Zhao-Yong, Zhang Yu-Jue, Meng Zhen, Li Jun, Meng Chun-Xiu, Qiang Hui-Fen, Hou Cai-Yao, Hou Lei, Liu Feng-Zhen, Zhang Bin
Department of Oral and Maxillofacial Surgery, School and Hospital of Stomatology, Shandong University and Shandong Provincial Key Laboratory of Oral Tissue Regeneration and Shandong Engineering Laboratory for Dental Materials and Oral Tissue Regeneration, Jinan, China.
Liaocheng People's Hospital, Medical College of Liaocheng University, Liaocheng, China.
Front Bioeng Biotechnol. 2022 Jan 5;9:801432. doi: 10.3389/fbioe.2021.801432. eCollection 2021.
The effective healing of a bone defect is dependent on the careful coordination of inflammatory and bone-forming cells. In the current work, pro-inflammatory M1 and anti-inflammatory M2 macrophages were co-cultured with primary murine bone mesenchymal stem cells (BMSCs), , to establish the cross-talk among polarized macrophages and BMSCs, and as well as their effects on osteogenesis. Meanwhile, macrophages influence the osteogenesis of BMSCs through paracrine forms such as exosomes. We focused on whether exosomes of macrophages promote osteogenic differentiation. The results indicated that M1 and M2 polarized macrophage exosomes all can promote osteogenesis of BMSCs. Especially, M1 macrophage-derived exosomes promote osteogenesis of BMSCs through microRNA-21a-5p at the early stage of inflammation. This research helps to develop an understanding of the intricate interactions among BMSCs and macrophages, which can help to improve the process of bone healing as well as additional regenerative processes by local sustained release of exosomes.
骨缺损的有效愈合依赖于炎症细胞和骨形成细胞的精确协调。在当前的研究中，将促炎性M1巨噬细胞和抗炎性M2巨噬细胞与原代小鼠骨髓间充质干细胞（BMSCs）共培养，以建立极化巨噬细胞与BMSCs之间的相互作用，以及它们对成骨的影响。同时，巨噬细胞通过外泌体等旁分泌形式影响BMSCs的成骨作用。我们聚焦于巨噬细胞外泌体是否促进成骨分化。结果表明，M1和M2极化巨噬细胞外泌体均能促进BMSCs的成骨作用。特别是，M1巨噬细胞来源的外泌体在炎症早期通过微小RNA-21a-5p促进BMSCs成骨。这项研究有助于深入了解BMSCs与巨噬细胞之间复杂的相互作用关系，这有助于通过外泌体的局部持续释放改善骨愈合过程以及其他再生过程。
