miR-26a-5p 通过抑制 PTGS2 表达抑制鼻咽癌进展。
miR-26a-5p suppresses nasopharyngeal carcinoma progression by inhibiting PTGS2 expression.
机构信息
Department of Otorhinolaryngology, Affiliated Puren Hospital of Wuhan University of Science and Technology, Wuhan, China.
Department of Pain Treatment, Affiliated Puren Hospital of Wuhan University of Science and Technology, Wuhan, China.
出版信息
Cell Cycle. 2022 Mar-Mar;21(6):618-629. doi: 10.1080/15384101.2022.2030168. Epub 2022 Jan 25.
Nasopharyngeal carcinoma (NPC) has a low five-year survival rate, and its pathogenesis remains unclear. There is an urgent need to improve our understanding of the genetic regulation of NPC tumorigenesis and development. The role of miR-26a-5p in NPC growth regulation and the expression of its target, PTGS2, was analyzed. Quantitative Real-time PCR assay was used to detect miR-26a-5p and PTGS2 expression in human NPC tissues and cell lines. The RNA pull-down dual-luciferase reporter assay was used to determine the association between miR-26a-5p and PTGS2. The effects of miR-26a-5p and PTGS2 on NPC cell viability, proliferation, migration, and invasion were measured by CCK-8, BrdU, and Transwell assays. miR-26a-5p expression in NPC tissues and cell lines was significantly decreased. The overexpression of miR-26a-5p inhibited the viability, proliferation, migration, and invasion of NPC cells. miR-26a-5p bound to the 3-'untranslated region of PTGS2, thus reducing PTGS2 protein levels. miR-26a-5p inhibited NPC development by reducing the expression of its target PTGS2.
鼻咽癌（NPC）五年生存率低，其发病机制尚不清楚。迫切需要提高我们对 NPC 肿瘤发生和发展的遗传调控的理解。分析了 miR-26a-5p 在 NPC 生长调节中的作用及其靶基因 PTGS2 的表达。定量实时 PCR 检测人 NPC 组织和细胞系中 miR-26a-5p 和 PTGS2 的表达。RNA 下拉双荧光素酶报告基因检测 miR-26a-5p 与 PTGS2 的相关性。CCK-8、BrdU 和 Transwell 测定 miR-26a-5p 和 PTGS2 对 NPC 细胞活力、增殖、迁移和侵袭的影响。miR-26a-5p 在 NPC 组织和细胞系中的表达明显降低。miR-26a-5p 的过表达抑制 NPC 细胞的活力、增殖、迁移和侵袭。miR-26a-5p 通过降低其靶基因 PTGS2 的表达来抑制 NPC 的发展。
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