Duan Bingyue, Shi Si, Yue Huijun, You Bo, Shan Ying, Zhu Ziyu, Bao Lili, You Yiwen
Department of Otorhinolaryngology Head and Neck Surgery, Affiliated Hospital of Nantong University, Nantong, Jiangsu Province, China.
Institute of Otorhinolaryngology Head and Neck Surgery, Affiliated Hospital of Nantong University, Nantong, Jiangsu Province, China.
J Cancer. 2019 Oct 22;10(26):6681-6692. doi: 10.7150/jca.30757. eCollection 2019.
The purpose of our study is to investigate the role of miR-17-5p in angiogenesis of nasopharyngeal carcinoma and the crosstalk between HUVECs and CNE-2 via exosomes. Firstly, flow cytometry, cell viability assay, transwell assay, and tube formation were used to explore the role of miR-17-5p in angiogenesis. Then zebrafish model was used to confirm effects of miR-17-5p on angiogenesis. qRT-PCR analysis and Immunofluorescence assay were used to explore the expression of miR-17-5p in NPC tissues and cells compared to the normal control. Besides, assays were used to analyze the biological functions of miR-17-5p in NPC. What's more, and assays were used to detect the function of exosomal miR-17-5p in angiogenesis. Finally, luciferase reporter assay and western bolt were used to determine the relationship between miR-17-5p and BAMBI. We observed that high expression of miR-17-5p promoted angiogenesis in NPC. Also, high expression of miR-17-5p promoted the NPC cells proliferation and migration. To know whether there's any communication between HUVECs and NPC cells, exosomes derived from CNE-2 cells were collected. Further results showed that exosomal miR-17-5p secreted from NPC promoted the angiogenesis. What's more, assays revealed that miR-17-5p targets BAMBI and regulates AKT/VEGF-A signaling. Our study showed that exosomal miR-17-5p derived from NPC cells promotes angiogenesis via targeting BAMBI and regulates AKT/VEGF-A signaling.
我们研究的目的是探讨miR-17-5p在鼻咽癌血管生成中的作用以及人脐静脉内皮细胞（HUVECs）与CNE-2细胞通过外泌体的相互作用。首先，采用流式细胞术、细胞活力测定、Transwell测定和管腔形成实验来探究miR-17-5p在血管生成中的作用。然后使用斑马鱼模型来证实miR-17-5p对血管生成的影响。与正常对照相比，采用qRT-PCR分析和免疫荧光测定来探究miR-17-5p在鼻咽癌组织和细胞中的表达。此外，采用实验分析miR-17-5p在鼻咽癌中的生物学功能。更重要的是，采用实验检测外泌体miR-17-5p在血管生成中的功能。最后，使用荧光素酶报告基因测定和蛋白质免疫印迹法来确定miR-17-5p与BAMBI之间的关系。我们观察到miR-17-5p的高表达促进了鼻咽癌中的血管生成。此外，miR-17-5p的高表达促进了鼻咽癌细胞的增殖和迁移。为了了解HUVECs与鼻咽癌细胞之间是否存在任何通讯，收集了来自CNE-2细胞的外泌体。进一步的结果表明，鼻咽癌分泌的外泌体miR-17-5p促进了血管生成。更重要的是，实验表明miR-17-5p靶向BAMBI并调节AKT/VEGF-A信号通路。我们的研究表明，源自鼻咽癌细胞的外泌体miR-17-5p通过靶向BAMBI促进血管生成并调节AKT/VEGF-A信号通路。
需注意，原文中部分表述不完整，有“ assays”这样未明确具体内容的表述，但按照要求完整翻译了给定文本。
