Gehlsen K R, Hendrix M J
Department of Anatomy, College of Medicine, University of Arizona, Tucson 85724.
Pigment Cell Res. 1987;1(1):16-21. doi: 10.1111/j.1600-0749.1987.tb00529.x.
An investigation of the invasiveness of avian neural crest cells and neural crest-derived melanocytes through a human amniotic basement membrane (BM) was undertaken. Avian neural tube explants or derived melanocyte populations were seeded directly onto BMs in membrane invasion culture system (MICS) chambers for periods of 24, 48, and 72 h. In 36 experimental trials for each group, neither neural crest nor neural crest-derived melanocytes were observed to have invaded the BMs. In concert with these studies, coculturing of B16F10 murine melanoma cells with avian neural crest-derived melanocytes was performed in MICS chambers. Under these experimental conditions, the neural crest-derived melanocytes were able to successfully invade the BMs and to a greater extent than the B16F10 tumor cells. These data suggest that neural crest cells and neural crest-derived melanocytes do not have the ability to invade the BM alone; however, they can be induced to be invasive when cocultured in the presence of B16F10 cells. Alternatively, the B16F10 cells may create weaknesses within the BM that facilitate migration of the pigmented crest cells.
对禽类神经嵴细胞和神经嵴衍生的黑素细胞通过人羊膜基底膜（BM）的侵袭性进行了研究。将禽类神经管外植体或衍生的黑素细胞群体直接接种到膜侵袭培养系统（MICS）小室中的基底膜上，培养24、48和72小时。在每组36次实验中，未观察到神经嵴或神经嵴衍生的黑素细胞侵入基底膜。与这些研究一致，在MICS小室中进行了B16F10小鼠黑色素瘤细胞与禽类神经嵴衍生的黑素细胞的共培养。在这些实验条件下，神经嵴衍生的黑素细胞能够成功侵入基底膜，且比B16F10肿瘤细胞侵入的程度更大。这些数据表明，神经嵴细胞和神经嵴衍生的黑素细胞本身没有侵入基底膜的能力；然而，当在B16F10细胞存在的情况下共培养时，它们可以被诱导具有侵袭性。或者，B16F10细胞可能在基底膜内造成薄弱点，便于色素嵴细胞迁移。
