被噬菌体P2整合抑制的大肠杆菌dnaA突变体中的染色体复制。
Chromosome replication in an Escherichia coli dnaA mutant integratively suppressed by prophage P2.
作者信息
Kuempel P L, Duerr S A, Maglothin P D
出版信息
J Bacteriol. 1978 Jun;134(3):902-12. doi: 10.1128/jb.134.3.902-912.1978.
Abstract
Escherichia coli CRT4624-P2sig5 is a dnaA mutant in which integration of the prophage P2sig5 has occurred at the attP2II site (min 85). This strain was integratively suppressed, and when cells were shifted to 42 degrees C replication was initiated at a site in or near the P2 prophage. Initially, this replication occurred primarily in the direction that corresponds to the clockwise direction on the genetic map. Replication also occurred in the counterclockwise direction, but the initiation of replication in this direction occurred approximately 40 min later than the initiation of replication in the other direction. Because of this delay, the replication forks that traveled in the clockwise direction were the first to arrive in the region of the replication terminus. These replication forks ceased replication near the aroD locus (min 37), and it is proposed that the replication terminus is between the aroD and rac loci (min 31). A model is proposed for the cycle of chromosome replication in this strain at 42 degrees C.
摘要
大肠杆菌CRT4624-P2sig5是一种dnaA突变体，其中原噬菌体P2sig5已整合到attP2II位点（85分钟处）。该菌株被整合抑制，当细胞转移到42摄氏度时，复制在P2原噬菌体内部或附近的一个位点起始。最初，这种复制主要发生在与遗传图谱上顺时针方向相对应的方向。复制也发生在逆时针方向，但这个方向的复制起始比另一个方向大约晚40分钟。由于这种延迟，沿顺时针方向移动的复制叉最先到达复制终点区域。这些复制叉在aroD基因座（37分钟处）附近停止复制，有人提出复制终点位于aroD和rac基因座之间（31分钟处）。提出了该菌株在42摄氏度时染色体复制周期的模型。
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本文引用的文献
1
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Virology. 1970 Oct;42(2):522-33. doi: 10.1016/0042-6822(70)90295-3.
2
The chromosome of Bacillus subtilis. I. Theory of marker frequency analysis.枯草芽孢杆菌的染色体。I. 标记频率分析理论
Genetics. 1965 Oct;52(4):747-57. doi: 10.1093/genetics/52.4.747.
3
On the process of cellular division in Escherichia coli: replication of the bacterial chromosome under control of prophage P2.关于大肠杆菌细胞分裂过程：原噬菌体P2控制下细菌染色体的复制。
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4
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