Lindahl G, Hirota Y, Jacob F
Proc Natl Acad Sci U S A. 1971 Oct;68(10):2407-11. doi: 10.1073/pnas.68.10.2407.
The temperature-sensitive mutant CRT46 of Escherichia coli K12 is unable to initiate new rounds of DNA replication at 42 degrees C. Mutants of bacteriophage P2 have been isolated, which, in the prophage state, allow mutant CRT46 to grow at 42 degrees C. The lysogenic bacteria that grow at 42 degrees C are apparently replicating under the control of prophage P2, which substitutes for the bacterial initiation system. The ability of prophage P2 cause this suppression phenomenon depends on the position of the prophage on the bacterial chromosome. Those lysogenic strains that are able to grow at 42 degrees C all carry the prophage close to metE. The P2 mutants that allow CRT46 to grow at 42 degrees C have insertions in the early region of the P2 genome. The suppression requires the cis-acting protein formed by gene A of P2.
大肠杆菌K12的温度敏感突变体CRT46在42℃时无法启动新一轮的DNA复制。已经分离出噬菌体P2的突变体，在原噬菌体状态下，这些突变体可使突变体CRT46在42℃生长。在42℃生长的溶原性细菌显然是在原噬菌体P2的控制下进行复制，原噬菌体P2替代了细菌起始系统。原噬菌体P2引起这种抑制现象的能力取决于原噬菌体在细菌染色体上的位置。那些能够在42℃生长的溶原性菌株都携带靠近metE的原噬菌体。使CRT46在42℃生长的P2突变体在P2基因组的早期区域有插入。这种抑制作用需要由P2基因A形成的顺式作用蛋白。
