rep突变。III. 复制中的大肠杆菌染色体的结构改变。
The rep mutation. III. Altered structure of the replicating Escherichia coli chromosome.
作者信息
Lane H E, Denhardt D T
出版信息
J Bacteriol. 1974 Nov;120(2):805-14. doi: 10.1128/jb.120.2.805-814.1974.
Abstract
The rep gene function of Escherichia coli is essential for the replication of P2 and phiX174 double-stranded deoxyribonucleic acid (DNA). Compared with isogenic rep(+) strains, rep mutants show the following characteristics: larger cell size, more DNA per cell, and a slightly lower DNA/mass ratio. The replicating rep chromosomes show a steeper gradient of marker frequencies and contain more replicating forks per chromosome. The nucleoid body of rep mutants sediments faster and contains more DNA. We deduce that the rep function is required for the "normal" replication of the E. coli chromosome and that in its absence the E. coli chromosome replicates in an altered manner, perhaps involving slower-moving replicating forks.
摘要
大肠杆菌的rep基因功能对于P2和φX174双链脱氧核糖核酸（DNA）的复制至关重要。与同基因的rep(+)菌株相比，rep突变体表现出以下特征：细胞体积更大、每个细胞中的DNA更多，以及DNA/质量比略低。正在复制的rep染色体显示出标记频率的梯度更陡，并且每条染色体包含更多的复制叉。rep突变体的类核体沉降更快且含有更多的DNA。我们推断rep功能是大肠杆菌染色体“正常”复制所必需的，并且在其缺失时，大肠杆菌染色体以改变的方式进行复制，可能涉及移动较慢的复制叉。
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