Tianjin Key Laboratory for Photoelectric Materials and Devices, School of Materials Science & Engineering, Tianjin University of Technology, Tianjin 300384, PR China.
Tianjin Key Laboratory for Photoelectric Materials and Devices, School of Materials Science & Engineering, Tianjin University of Technology, Tianjin 300384, PR China; School of Materials Science and Engineering, Harbin Institute of Technology, Harbin 150001, PR China.
Spectrochim Acta A Mol Biomol Spectrosc. 2022 Apr 15;271:120917. doi: 10.1016/j.saa.2022.120917. Epub 2022 Jan 20.
Considering the scarcity of hydrogen sulfide (HS) probes with subcellular organelle targeting, especially probes with near-infrared (NIR) emission wavelengths fluorophores, our group has been working to overcome this problem and looking forward to providing potential practical tools for exploring the relationship between the physiology and pathology of HS at subcellular level. In this paper, a novel colorimetric and NIR fluorescent probe SHCy-HS for the specific detection of HS in lysosome over other biological thiols was designed and synthesized. The xanthene-benzothiozolium fluorophore was chosen to provide fluorescence emission maxima over 735 nm, and 2,4-dinitrophenyl group was chosen as fluorescence quenching group and specific HS response site. Impressively, SHCy-HS exhibited high selectivity, fast response and detection limit as low as 0.116 μM for HS, marked obvious color changes in naked-eye and fluorescence. Specially, SHCy-HS was capable of specifically imaging endogenous lysosomal hydrogen sulfide, providing a potential tool for exploring the function of HS at subcellular level.
鉴于具有亚细胞细胞器靶向的硫化氢 (HS) 探针，特别是具有近红外 (NIR) 发射波长荧光团的探针非常稀缺，我们小组一直在努力克服这一问题，并希望为探索 HS 在亚细胞水平的生理学和病理学之间的关系提供潜在的实用工具。在本文中，设计并合成了一种新型比色法和近红外荧光探针 SHCy-HS，用于特异性检测溶酶体中的 HS，而其他生物硫醇则不行。选择了呫吨-苯并噻唑荧光团，以提供超过 735nm 的荧光发射最大值，而 2,4-二硝基苯基基团则被选为荧光猝灭基团和特定的 HS 反应位点。令人印象深刻的是，SHCy-HS 对 HS 表现出高选择性、快速响应和检测限低至 0.116μM，在裸眼和荧光下均明显发生颜色变化。特别是，SHCy-HS 能够特异性地对内源性溶酶体硫化氢进行成像，为探索 HS 在亚细胞水平的功能提供了一种潜在的工具。
