School of Pharmacy, Yancheng Teachers University, Yancheng 224051, China.
Molecules. 2024 May 19;29(10):2386. doi: 10.3390/molecules29102386.
Monitoring hydrogen sulfide (HS) in living organisms is very important because HS acts as a regulator in many physiological and pathological processes. Upregulation of endogenous HS concentration has been shown to be closely related to the occurrence and development of tumors, atherosclerosis, neurodegenerative diseases and diabetes. Herin, a novel fluorescent probe with aggregation-induced emission was designed. Impressively, exhibited a high selectivity, fast response (1 min) and low detection limit (0.61 μM) for HS in PBS buffer (10 mM, pH = 7.42). Moreover, the reaction mechanism between and HS was conducted by Job's plot, HR-MS, and DFT. In particular, was successfully employed to detect HS in HeLa cells.
监测生物体内的硫化氢（HS）非常重要，因为 HS 在许多生理和病理过程中充当调节剂。内源性 HS 浓度的上调已被证明与肿瘤、动脉粥样硬化、神经退行性疾病和糖尿病的发生和发展密切相关。在此，设计了一种具有聚集诱导发射的新型荧光探针。令人印象深刻的是，在 PBS 缓冲液（10 mM，pH = 7.42）中， 对 HS 表现出高选择性、快速响应（1 分钟）和低检测限（0.61 μM）。此外，HS 与 的反应机制通过 Job 图、高分辨率质谱（HR-MS）和密度泛函理论（DFT）进行了研究。特别是， 成功地用于检测 HeLa 细胞中的 HS。
