College of Biosystems Engineering and Food Science, Zhejiang University, Hangzhou, Zhejiang 310058, China.
Ningbo Research Institute, Zhejiang University, Ningbo 315100, China.
ACS Appl Mater Interfaces. 2022 Feb 23;14(7):8680-8692. doi: 10.1021/acsami.1c19754. Epub 2022 Jan 27.
Covalent organic frameworks (COFs) possess fascinating features that have sparked increasing interest as drug carriers in biomedical applications. However, the promising properties of COFs in wound healing have rarely been reported. Herein, a facile one-pot method is reported to prepare a curcumin-loaded COF (CUR@COF) by the condensation reaction and the Schiff base reaction and to further incorporate CUR@COF into polycaprolactone (PCL) nanofibrous membranes (CUR@COF/PCL NFMs) through electrospinning to develop a pH-triggered drug release platform for wound dressing. CUR@COF has a high CUR loading capacity of 27.68%, and CUR@COF/PCL NFMs exhibit increased thermal stability, improved mechanical properties, good biocompatibility, and enhanced antibacterial and antioxidant activities. More importantly, CUR@COF-based membranes show a pH-responsive CUR release profile by protonation under acidic conditions, suggesting the promotion of CUR release from membranes under an acidic extracellular microenvironment. The histopathological analysis and immunofluorescence staining of an in vivo skin defect model indicate that CUR@COF/PCL NFMs can accelerate wound healing and skin regeneration by reducing the expression of inflammatory factors (TNF-α) and enhancing the expression of angiogenesis (VEGF). This work provides a new strategy by employing COF-based drug-encapsulated nanocomposites for wound dressing applications.
共价有机骨架（COFs）具有迷人的特性，作为药物载体在生物医学应用中引起了越来越多的关注。然而，COFs 在伤口愈合方面的有前途的特性很少有报道。在此，报道了一种通过缩合反应和希夫碱反应制备载姜黄素 COF（CUR@COF）的简便一锅法，并通过静电纺丝将 CUR@COF 进一步掺入聚己内酯（PCL）纳米纤维膜（CUR@COF/PCL NFMs）中，以开发用于伤口敷料的 pH 触发药物释放平台。CUR@COF 具有高达 27.68%的 CUR 负载能力，CUR@COF/PCL NFMs 表现出增强的热稳定性、改善的机械性能、良好的生物相容性以及增强的抗菌和抗氧化活性。更重要的是，基于 CUR@COF 的膜在酸性条件下通过质子化显示出 pH 响应的 CUR 释放特性，表明在酸性细胞外微环境下促进了膜中 CUR 的释放。体内皮肤缺损模型的组织病理学分析和免疫荧光染色表明，CUR@COF/PCL NFMs 可以通过减少炎症因子（TNF-α）的表达和增强血管生成（VEGF）来加速伤口愈合和皮肤再生。这项工作通过使用基于 COF 的载药纳米复合材料为伤口敷料应用提供了一种新策略。
