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N10-甲酰四氢叶酸是大肠杆菌中甘氨酰胺核糖核苷酸转甲酰酶的甲酰基供体。

N10-Formyltetrahydrofolate is the formyl donor for glycinamide ribotide transformylase in Escherichia coli.

作者信息

Dev I K, Harvey R J

出版信息

J Biol Chem. 1978 Jun 25;253(12):4242-4.

PMID:350869
Abstract

Glycinamide ribotide transformylase from Escherichia coli was obtained free of N5,N10-methenyltetrahydrofolate cyclohydrolase activity by DEAE-cellulose chromatography. In reaction mixtures containing this enzyme preparation in potassium maleate buffer, pH 7.2, no detectable interconversion of N5,N10-methenyltetrahydrofolate occurred. Upon addition of glycinamide ribotide, N-formylglycinamide ribotide was formed when N10-formyltetrahydrofolate was present; no formylation occurred in the presence of N5,N10-methenyltetrahydrofolate. A method for the synthesis and purification of glycinamide ribotide is presented.

摘要

通过二乙氨基乙基纤维素色谱法获得了不含N5,N10-亚甲基四氢叶酸环水解酶活性的大肠杆菌甘氨酰胺核糖核苷酸转甲酰基酶。在含有该酶制剂的马来酸钾缓冲液（pH 7.2）反应混合物中，未检测到N5,N10-亚甲基四氢叶酸的相互转化。加入甘氨酰胺核糖核苷酸后，当存在N10-甲酰四氢叶酸时会形成N-甲酰甘氨酰胺核糖核苷酸；在N5,N10-亚甲基四氢叶酸存在的情况下不会发生甲酰化反应。本文介绍了一种甘氨酰胺核糖核苷酸的合成及纯化方法。

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N10-Formyltetrahydrofolate is the formyl donor for glycinamide ribotide transformylase in Escherichia coli.N10-甲酰四氢叶酸是大肠杆菌中甘氨酰胺核糖核苷酸转甲酰酶的甲酰基供体。
J Biol Chem. 1978 Jun 25;253(12):4242-4.
2
A complex of N5,N10-methylenetetrahydrofolate dehydrogenase and N5,N10-methenyltetrahydrofolate cyclohydrolase in Escherichia coli. Purification, subunit structure, and allosteric inhibition by N10-formyltetrahydrofolate.大肠杆菌中N5,N10-亚甲基四氢叶酸脱氢酶和N5,N10-亚甲基四氢叶酸环水解酶复合物。纯化、亚基结构以及N10-甲酰四氢叶酸的变构抑制作用。
J Biol Chem. 1978 Jun 25;253(12):4245-53.
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L(-)-10-Formyltetrahydrofolate is the cofactor for glycinamide ribonucleotide transformylase from chicken liver.L(-)-10-甲酰四氢叶酸是鸡肝中甘氨酰胺核糖核苷酸转甲酰酶的辅因子。
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