Smith G K, Benkovic P A, Benkovic S J
Biochemistry. 1981 Jul 7;20(14):4034-6. doi: 10.1021/bi00517a013.
It is shown that L(-)-10-formyltetrahydrofolate serves as the cofactor for glycinamide ribonucleotide transformylase from chicken liver. The utilization of L(-)-10-formyl-H4folate was not previously recognized, because L-(+)-10-formyl-H4folate is an excellent competitive inhibitor of the enzyme, Ki = 0.75 +/- 0.07 microM, and historically the transformylase assay was carried out with a mixture of diastereomers. The results are discussed in relation to the utilization of L(+)-5,10-methenyl-H4folate.
已表明L(-)-10-甲酰四氢叶酸作为鸡肝甘氨酰胺核糖核苷酸转甲酰酶的辅因子。以前未认识到L(-)-10-甲酰-H4叶酸的利用，因为L-(+)-10-甲酰-H4叶酸是该酶的一种出色的竞争性抑制剂，Ki = 0.75 +/- 0.07 microM，并且历史上转甲酰酶测定是用非对映异构体混合物进行的。结合L(+)-5,10-亚甲基-H4叶酸的利用对结果进行了讨论。
