Department of Health Sciences, Federal Rural University of the Semi-Arid (UFERSA), Mossoró, Brazil.
Department of Morphology, Federal University of Rio Grande do Norte (UFRN), Natal, Brazil.
Ann Anat. 2022 Apr;241:151892. doi: 10.1016/j.aanat.2022.151892. Epub 2022 Jan 25.
Cardiomyocyte size histomorphometry is widely required in myocardial remodeling research, but it relies on subjective, time-consuming manual tracing. We aimed to present the CmyoSize, a Fiji/ImageJ macro that reproducibly measures the transnuclear cross-sectional size of cardiomyocytes in multiple H&E images.
CmyoSize output per image are: mean cross-sectional area (CSA), CSA's local standard deviation (LSD), mean width, and width's LSD. CmyoSize was validated against measurements of an expert examiner, using myocardium micrographs (400-fold magnification) from rats treated with hyperbaric oxygen (HBO), streptozotocin (STZ), as well as control rats (five images/heart, five rats/group).
CmyoSize achieved high cardiomyocyte detection precision (95.17 ± 1.11%) and it analyzed each image at least 30-times faster than expert examiner (8.44 ± 0.17 s vs. 266.70 ± 25.15 s). Bland-Altman analyses showed no meaningful bias ( ± 95% confidence interval) between both methods: mean CSA = 1.88 ± 6.83 µm, CSA's LSD = -4.55 ± 5.07 µm; mean width = -0.02 ± 0.22 µm; width's LSD = -0.16 ± 0.17 µm. Pearson's r ranged from 0.85 to 0.94 (P < 0.0001). CmyoSize revealed homogenous cardiomyocyte hypertrophy in HBO-treated animals and STZ-induced heterogeneous hypertrophy.
Fully automated, standardized cardiomyocyte size quantification in H&E images using CmyoSize is feasible, accurate, and time-efficient.
心肌重构研究中广泛需要心肌细胞大小形态计量学，但它依赖于主观、耗时的手动追踪。我们旨在介绍 CmyoSize，这是一个 Fiji/ImageJ 宏，可在多个 H&E 图像中重现测量心肌细胞的跨核横截面大小。
CmyoSize 为每张图像输出：平均横截面积（CSA）、CSA 的局部标准差（LSD）、平均宽度和宽度的 LSD。使用高压氧（HBO）、链脲佐菌素（STZ）处理以及对照大鼠（每组 5 只大鼠，每张心脏 5 张图像）的心肌显微照片（400 倍放大率）对 CmyoSize 进行了验证。
CmyoSize 实现了高心肌细胞检测精度（95.17±1.11%），并且比专家检查者分析每张图像至少快 30 倍（8.44±0.17 秒与 266.70±25.15 秒）。Bland-Altman 分析表明两种方法之间没有有意义的偏差（±95%置信区间）：平均 CSA=1.88±6.83µm，CSA 的 LSD=-4.55±5.07µm；平均宽度=-0.02±0.22µm；宽度的 LSD=-0.16±0.17µm。Pearson's r 范围从 0.85 到 0.94（P<0.0001）。CmyoSize 显示 HBO 处理动物的心肌细胞均匀肥大和 STZ 诱导的不均匀肥大。
使用 CmyoSize 在 H&E 图像中进行全自动、标准化的心肌细胞大小定量是可行、准确和高效的。
