Peng Tianfei, Liu Xuemei, Tian Fayi, Xu Hongfei, Yang Fengting, Chen Xuewei, Gao Xiwu, Lv Yuntong, Li Jianyi, Pan Yiou, Shang Qingli
College of Plant Science, Jilin University, Changchun, China.
School of Agricultural Science, Zhengzhou University, Zhengzhou, China.
Pest Manag Sci. 2022 May;78(5):1982-1991. doi: 10.1002/ps.6818. Epub 2022 Feb 10.
Spirotetramat is a tetramic acid derivative insecticide with novel modes of action for controlling Aphis gossypii Glover in the field. Previous studies have shown that long noncoding RNAs (lncRNAs) and cytochrome P450 monooxygenases (P450s) are involved in the detoxification process. However, the functions of lncRNAs in regulating P450 gene expression in spirotetramat resistance in A. gossypii are unknown.
In this study, we found CYP4CJ1, CYP6CY7 and CYP6CY21 expression levels to be significantly upregulated in a spirotetramat-resistant (SR) strain compared with a susceptible (SS) strain. Furthermore, knockdown of CYP4CJ1, CYP6CY7 and CYP6CY21 increased nymph and adult mortality in the SR strain following exposure to spirotetramat. Drosophila ectopically expressing CYP380C6, CYP4CJ1, CYP6DA2, CYP6CY7 and CYP6CY21 showed significantly decreased mortality after spirotetramat exposure, and CYP380C6, CYP4CJ1 and CYP6CY21 are putative targets of six lncRNAs. Silencing of lncRNAs MSTRG.36649.2/5 and MSTRG.71880.1 changed CYP6CY21 and CYP380C6 expression, altering the sensitivity of the SR strain to spirotetramat. Moreover, MSTRG.36649.2/5 did not compete for microRNA (miRNA) binding to regulate CYP6CY21 expression.
Our results confirm that CYP380C6, CYP4CJ1, CYP6DA2, CYP6CY7 and CYP6CY21 are potentially involved in the development of spirotetramat resistance in A. gossypii, and MSTRG.36649.2/5 and MSTRG.71880.1 probably regulate CYP6CY21 and CYP380C6 expression other than through the "sponge effect" of competing for miRNA binding. Our results provide a favorable molecular basis for studying cotton aphid P450 genes and lncRNA functions in spirotetramat resistance development.
螺虫乙酯是一种具有新型作用模式的四元酸衍生物杀虫剂，用于田间防治棉蚜。先前的研究表明，长链非编码RNA（lncRNAs）和细胞色素P450单加氧酶（P450s）参与解毒过程。然而，lncRNAs在棉蚜对螺虫乙酯抗性中调节P450基因表达的功能尚不清楚。
在本研究中，我们发现与敏感（SS）品系相比，在螺虫乙酯抗性（SR）品系中CYP4CJ1、CYP6CY7和CYP6CY21的表达水平显著上调。此外，敲低CYP4CJ1、CYP6CY7和CYP6CY21会增加SR品系若虫和成虫在接触螺虫乙酯后的死亡率。异位表达CYP380C6、CYP4CJ1、CYP6DA2、CYP6CY7和CYP6CY21的果蝇在接触螺虫乙酯后死亡率显著降低，且CYP380C6、CYP4CJ1和CYP6CY21是6种lncRNAs的假定靶标。lncRNAs MSTRG.36649.2/5和MSTRG.71880.1的沉默改变了CYP6CY21和CYP380C6的表达，改变了SR品系对螺虫乙酯的敏感性。此外，MSTRG.36649.2/5并不通过竞争微小RNA（miRNA）结合来调节CYP6CY21的表达。
我们的结果证实CYP380C6、CYP4CJ1、CYP6DA2、CYP6CY7和CYP6CY21可能参与棉蚜对螺虫乙酯抗性的形成，且MSTRG.36649.2/5和MSTRG.71880.1可能不是通过竞争miRNA结合的“海绵效应”来调节CYP6CY21和CYP380C6的表达。我们的结果为研究棉蚜P450基因和lncRNA在螺虫乙酯抗性形成中的功能提供了有利的分子基础。
