College of Plant Science, Jilin University, Changchun 130062, P.R. China.
Department of Entomology, China Agricultural University, Beijing 100193, P.R. China.
J Agric Food Chem. 2021 Jun 2;69(21):5849-5857. doi: 10.1021/acs.jafc.1c00922. Epub 2021 May 20.
Cyantraniliprole targets the ryanodine receptor and shows cross-spectrum activity against a broad range of chewing and sucking pests. In this study, a cyantraniliprole-resistant cotton aphid strain (CyR) developed resistance 17.30-fold higher than that of a susceptible (SS) strain. Bioassay results indicated that CyR developed increased cross-resistance to cyfluthrin, α-cypermethrin, imidacloprid, and acephate. In CyR, piperonyl butoxide synergistically increased the toxicity of cyantraniliprole, α-cypermethrin, and cyfluthrin. The cytochrome P450 activities in the CyR strain were significantly higher than those in the SS strain. The mRNA expression of , , , , , and in the CYP3 clade, and , , , , and in the CYP4 clade, was significantly higher in CyR than in SS. The depletion of the most abundant transcript by RNAi also significantly increased the sensitivity of CyR to cyantraniliprole. Transgenic expression of , , , and in suggested that the expression of and was sufficient to confer cyantraniliprole resistance, with being the most effective, and that , , and were related to α-cypermethrin cross-resistance. These results indicate the involvement of P450 genes in cyantraniliprole resistance and pyrethroid cross-resistance and provide an overall view of the metabolic factors involved in resistance development.
氯虫苯甲酰胺作用于兰尼碱受体，对广泛的咀嚼式和刺吸式害虫具有广谱活性。在本研究中，一种对氯虫苯甲酰胺具有 17.30 倍抗性的棉蚜抗性品系（CyR）比敏感品系（SS）的抗性高 17.30 倍。生物测定结果表明，CyR 对氯氟氰菊酯、α-氯氰菊酯、吡虫啉和乙酰甲胺磷的交叉抗性增加。在 CyR 中，增效醚协同增加了氯虫苯甲酰胺、α-氯氰菊酯和氯氟氰菊酯的毒性。CyR 菌株的细胞色素 P450 活性明显高于 SS 菌株。CYP3 类群中的 、 、 、 、 和 CYP4 类群中的 、 、 、 、 和 基因在 CyR 中的 mRNA 表达明显高于 SS。通过 RNAi 耗竭最丰富的 转录本也显著增加了 CyR 对氯虫苯甲酰胺的敏感性。在 中过表达 、 、 和 表明 和 的表达足以赋予对氯虫苯甲酰胺的抗性，其中 最有效，而 、 和 与 α-氯氰菊酯的交叉抗性有关。这些结果表明 P450 基因参与了氯虫苯甲酰胺抗性和拟除虫菊酯类交叉抗性，为抗性发展涉及的代谢因素提供了全面的认识。
