Jambwa Prosper, Makhubu Fikile N, Matope Gift, Fouche Gerda, McGaw Lyndy J
Phytomedicine Programme, Department of Paraclinical Sciences, University of Pretoria, Onderstepoort, South Africa.
Department of Veterinary Biosciences, University of Zimbabwe, Harare, Zimbabwe.
Front Vet Sci. 2022 Jan 13;8:800272. doi: 10.3389/fvets.2021.800272. eCollection 2021.
There has been burgeoning interest in plant-based feed additives following restrictions placed on the use of antibiotic feed additives in many countries. Phytogenic feed additives are recommended to have a range of useful properties to support the growth and development of poultry to a similar level as that obtained by supplementing feed with antibiotics. The aim of this study was to evaluate the antibacterial, anti-lipoxygenase and antioxidant activity, and safety of fractions and isolated compounds from leaves of . Antibacterial activities of the fractions and isolated compounds were determined against a panel of bacteria using a two-fold serial microdilution assay and qualitative bioautography assays. Anti-lipoxygenase activity was evaluated using the ferrous oxidation-xylenol orange (FOX) method. Antioxidant activity was assessed qualitatively and quantitatively using radical scavenging assays. Dichloromethane and ethyl acetate fractions from solvent-solvent partitioning had the best antibacterial activity with MIC values ranging from 156 to 313 μg/ml. Fractions obtained from column chromatography had significant to weak antibacterial activity with MIC values ranging from 50 to 1,250 μg/ml. Bioautography showed clear bands of bacterial inhibition, indicating the presence of a number of active compounds in several fractions. The ethyl acetate fraction and all the tested column fractions had potent anti-lipoxygenase activity with IC values of ≤2.5 μg/ml which were lower than that of quercetin (positive control), indicating anti-inflammatory potential. The ethyl acetate fraction and several column fractions had powerful antioxidant activity with IC values of ≤5 μg/ml in the ABTS assay. Cytotoxicity values against Vero kidney cells ranged from LC = 40.0-989.3 μg/ml. Bioassay-guided fractionation led to the isolation and identification of a known bioactive compound, luteolin. is a promising candidate for the development of poultry phytogenic feed additives.
在许多国家对抗生素饲料添加剂的使用进行限制之后，人们对植物性饲料添加剂的兴趣迅速增长。植物源饲料添加剂被认为具有一系列有益特性，能够将家禽的生长发育维持在与添加抗生素的饲料相似的水平。本研究的目的是评估[植物名称]叶片提取物及其分离化合物的抗菌、抗脂氧合酶和抗氧化活性以及安全性。采用两倍系列微量稀释法和定性生物自显影法，测定提取物及其分离化合物对一组细菌的抗菌活性。使用亚铁氧化-二甲苯酚橙（FOX）法评估抗脂氧合酶活性。通过自由基清除试验对抗氧化活性进行定性和定量评估。溶剂-溶剂分配法得到的二氯甲烷和乙酸乙酯提取物具有最佳抗菌活性，最低抑菌浓度（MIC）值在156至313μg/ml之间。柱色谱法得到的提取物具有显著至微弱的抗菌活性，MIC值在50至1250μg/ml之间。生物自显影显示出明显的细菌抑制带，表明几个提取物中存在多种活性化合物。乙酸乙酯提取物和所有测试的柱层析提取物具有较强的抗脂氧合酶活性，IC值≤2.5μg/ml，低于槲皮素（阳性对照），表明具有抗炎潜力。在ABTS试验中，乙酸乙酯提取物和几个柱层析提取物具有强大的抗氧化活性，IC值≤5μg/ml。对非洲绿猴肾细胞的细胞毒性值范围为LC = 40.0 - 989.3μg/ml。生物活性导向分离法导致分离并鉴定出一种已知的生物活性化合物木犀草素。[植物名称]是开发家禽植物源饲料添加剂的一个有前景的候选物。
