白藜芦醇通过线粒体自噬改善脱氧雪腐镰刀菌烯醇引发的肠道损伤及…… （原文结尾不完整）
Resveratrol Ameliorates Intestinal Damage Challenged With Deoxynivalenol Through Mitophagy and .
作者信息
Huang Yujian, Zheng Changbing, Song Bo, Wang Li, Xiao Hao, Jiang Zongyong
机构信息
State Key Laboratory of Livestock and Poultry Breeding, Ministry of Agriculture Key Laboratory of Animal Nutrition and Feed Science in South China, Guangdong Public Laboratory of Animal Breeding and Nutrition, Guangdong Provincial Key Laboratory of Animal Breeding and Nutrition, Maoming Branch, Guangdong Laboratory for Lingnan Modern Agriculture, Institute of Animal Science, Guangdong Academy of Agricultural Sciences, Guangzhou, China.
Guangdong Provincial Key Laboratory of Animal Nutrition Control, Institute of Subtropical Animal Nutrition and Feed, College of Animal Science, South China Agricultural University, Guangzhou, China.
出版信息
Front Vet Sci. 2022 Jan 13;8:807301. doi: 10.3389/fvets.2021.807301. eCollection 2021.
Deoxynivalenol (DON) reduces growth performance and damage intestinal function, and resveratrol (RES) has positive effects on growth performance and intestinal function. The purpose of this study was to investigate the protective mechanism of RES and challenged with DON. The results showed that dietary supplementation with DON significantly increase the mRNA expression levels of mitophagy- related genes, and protein level for PINK1, Parkin, Beclin-1, Lamp, Atg5, Map1lc, Bnip3, Fundc1, Bcl2l1 and SQSTMS1 ( < 0.05), while supplementation with both RES and DON decreased those indexes in the ileum. Besides DON significantly decreased protein level for Pyruvate Dehydrogenase, Cytochrome c, MFN1, OPA1, and PHB1 ( < 0.05), while supplementation with both RES and DON increased protein level for PHB1, SDHA, and VDAC in the ileum. Moreover, , we found that DON significantly decreased mitochondrial respiration ( < 0.05), while RES + DON increased the rate of spare respiratory capacity. Also, DON significantly decreased total NAD and ATP ( < 0.05), while RES + DON increased the total NAD and ATP. These results indicate that RES may ameliorates the intestinal damage challenged with deoxynivalenol through mitophagy in weaning piglets.
脱氧雪腐镰刀菌烯醇（DON）会降低生长性能并损害肠道功能，而白藜芦醇（RES）对生长性能和肠道功能具有积极作用。本研究的目的是探讨RES对DON攻击的保护机制。结果表明，日粮中添加DON显著提高了线粒体自噬相关基因的mRNA表达水平，以及PINK1、Parkin、Beclin-1、Lamp、Atg5、Map1lc、Bnip3、Fundc1、Bcl2l1和SQSTMS1的蛋白水平（P<0.05），而同时添加RES和DON则降低了回肠中的这些指标。此外，DON显著降低了丙酮酸脱氢酶、细胞色素c、MFN1、OPA1和PHB1的蛋白水平（P<0.05），而同时添加RES和DON则提高了回肠中PHB1、SDHA和VDAC的蛋白水平。此外，我们发现DON显著降低了线粒体呼吸（P<0.05），而RES+DON提高了备用呼吸能力的速率。同时，DON显著降低了总NAD和ATP（P<0.05），而RES+DON提高了总NAD和ATP。这些结果表明，RES可能通过线粒体自噬改善断奶仔猪脱氧雪腐镰刀菌烯醇攻击所致的肠道损伤。
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