Institute of Subtropical Agriculture, Chinese Academy of Sciences, Changsha, Hunan 410125, China.
University of Chinese Academy of Sciences, Beijing 100008, China.
Oxid Med Cell Longev. 2020 Jan 22;2020:5363546. doi: 10.1155/2020/5363546. eCollection 2020.
The present study was performed to evaluate the antioxidant and intestinal protective effects of baicalin-copper on deoxynivalenol-challenged piglets. Forty weaned piglets were randomly divided into four groups and assigned to different diets: (1) basal diet (Con), (2) 4 mg/kg deoxynivalenol of basal diet (DON), (3) 5 g/kg baicalin-copper of basal diet (BCU); and (4) 4 mg/kg deoxynivalenol + 5 g/kg baicalin-copper of basal diet (DBCU). The results showed that the ADFI and ADG of piglets in the DON group were markedly lower than those in the Con group, but the ADFI and ADG of the DBCU group were not significantly different from those of the Con group. In piglets fed a DON-contaminated diet, dietary supplementation with BCU significantly decreased the mRNA levels of P70S6K, 4E-BP1, and HSP70 in the liver, the protein expression of HO-1 in the jejunum, and the expression of p-Nrf2 and p-NF-B in the ileum but increased Mn-SOD activity in serum. Dietary supplementation with BCU increased jejunal maltase, ZIP4 and MT mRNA levels, and serum concentrations of Arg, Val, Ile, Leu, Lys, and Tyr in DON-contaminated piglets. In summary, BCU can alleviate the growth impairment induced by DON and enhance antioxidant capacity and nutrition absorption in piglets fed DON-contaminated diets.
本研究旨在评估黄芩苷铜对脱氧雪腐镰刀菌烯醇（DON）挑战仔猪的抗氧化和肠道保护作用。将 40 头断奶仔猪随机分为 4 组，并分配到不同的日粮中：（1）基础日粮（Con）；（2）基础日粮添加 4mg/kg DON（DON）；（3）基础日粮添加 5g/kg 黄芩苷铜（BCU）；（4）基础日粮添加 4mg/kg DON+5g/kg 黄芩苷铜（DBCU）。结果表明，DON 组仔猪的 ADFI 和 ADG 明显低于 Con 组，但 DBCU 组的 ADFI 和 ADG 与 Con 组无显著差异。在饲喂 DON 污染日粮的仔猪中，BCU 的添加显著降低了肝脏中 P70S6K、4E-BP1 和 HSP70 的 mRNA 水平，空肠中 HO-1 的蛋白表达，回肠中 p-Nrf2 和 p-NF-B 的表达，但增加了血清中 Mn-SOD 活性。BCU 的添加增加了受 DON 污染仔猪空肠中麦芽糖酶、ZIP4 和 MT 的 mRNA 水平以及血清中 Arg、Val、Ile、Leu、Lys 和 Tyr 的浓度。综上所述，BCU 可缓解 DON 引起的生长抑制，增强受 DON 污染日粮喂养仔猪的抗氧化能力和营养吸收。
