Infectious Diseases and Tropical Medicine Research Center, Research Institute of Cellular and Molecular Sciences in Infectious Diseases, Zahedan University of Medical Sciences, Zahedan, Iran.
Department of Parasitology and Mycology, Faculty of Medicine, Zahedan University of Medical Sciences, Zahedan, Iran.
Am J Trop Med Hyg. 2022 Jan 31;106(3):841-845. doi: 10.4269/ajtmh.21-0970.
Malaria is one of the most serious health problems in many countries, including Iran. Accurate diagnosis is important regardless of the elimination status of a country. A cross-sectional study was performed on 105 people who were suspected to be positive for malaria infection in Sistan and Baluchistan, Iran. Blood smears (thin and thick films) were stained with 10% Giemsa. DNA was extracted from the prepared thin and thick films for molecular methods. Multiplex/nested polymerase chain reaction (mn-PCR), loop-mediated isothermal amplification (LAMP), and light microscopy (LM) were compared with nested PCR (nPCR) as a gold standard. Of 105 subjects, 52 (49.5%), 58 (55.2%), 58 (55.2%), and 63 (60%) were positive for malaria by LM, nPCR, mn-PCR, and LAMP, respectively. The sensitivity, specificity, and kappa were 92.1%, 100%, and 0.9 for LAMP and 100%, 100%, and 1 for mn-PCR, respectively. Eight cases of coinfection (Plasmodium vivax and Plasmodium falciparum) that were not detected by LM method were diagnosed by mn-PCR and LAMP. In the present study, the high sensitivity and specificity of LAMP and mn-PCR indicate that these two tests are good alternatives to nPCR for malaria diagnosis.
疟疾是包括伊朗在内的许多国家面临的最严重的健康问题之一。无论一个国家的疟疾消除状况如何，准确诊断都很重要。本研究对伊朗锡斯坦和俾路支省 105 名疑似疟疾感染的患者进行了横断面研究。血涂片（薄血膜和厚血膜）用 10%吉氏染色。从制备的薄血膜和厚血膜中提取 DNA 进行分子方法检测。多重/嵌套聚合酶链反应（mn-PCR）、环介导等温扩增（LAMP）和光镜（LM）与巢式 PCR（nPCR）作为金标准进行比较。105 名受试者中，52 名（49.5%）、58 名（55.2%）、58 名（55.2%）和 63 名（60%）分别通过 LM、nPCR、mn-PCR 和 LAMP 检测为疟疾阳性。LAMP 的灵敏度、特异性和 Kappa 值分别为 92.1%、100%和 0.9，mn-PCR 的灵敏度、特异性和 Kappa 值分别为 100%、100%和 1。8 例通过 LM 方法未检测到的混合感染（间日疟原虫和恶性疟原虫）通过 mn-PCR 和 LAMP 诊断。本研究中，LAMP 和 mn-PCR 的高灵敏度和特异性表明，这两种检测方法是 nPCR 诊断疟疾的良好替代方法。
