Bard J, Levitt D
Clin Immunol Immunopathol. 1986 Feb;38(2):150-60. doi: 10.1016/0090-1229(86)90134-0.
We have previously shown that infants with pneumonitis caused by Chlamydia trachomatis, an obligate intracellular bacterium, possess increased percentages of B lymphocytes but not T lymphocytes in their peripheral blood. It was then demonstrated that chlamydiae induce proliferation in vitro of human peripheral blood B lymphocytes and, in the presence of T cells, differentiation of B cells to immunoglobulin-secreting cells. In this study, we show that C. trachomatis (L2 serovar) binds preferentially to 50% of human B lymphocytes from peripheral blood but only to a small percentage, if any, of T cells. Both monocytes and granulocytes bind and ingest chlamydiae. Despite chlamydial binding to B cells and ingestion by monocytes, no uptake by B cells and limited growth (fewer than 0.5% inclusion-containing cells) in monocytes occur. There is a dramatic decrease in the percentage of cells associated with the bacteria after culture. These results are the first demonstration of binding of C. trachomatis (L2 serovar) to lymphocytes and represent a direct step toward correlating physical interactions between bacteria and lymphocytes with specific immunostimulatory activities in vitro.
我们之前已经表明，由沙眼衣原体（一种专性细胞内细菌）引起肺炎的婴儿，其外周血中B淋巴细胞的百分比增加，而T淋巴细胞的百分比未增加。随后证明，衣原体可诱导人外周血B淋巴细胞在体外增殖，并且在T细胞存在的情况下，可使B细胞分化为免疫球蛋白分泌细胞。在本研究中，我们发现沙眼衣原体（L2血清型）优先结合50%的外周血人B淋巴细胞，但仅结合一小部分（如果有的话）T细胞。单核细胞和粒细胞均可结合并摄取衣原体。尽管衣原体可与B细胞结合并被单核细胞摄取，但B细胞不摄取，且在单核细胞中生长受限（含包涵体细胞少于0.5%）。培养后与细菌相关的细胞百分比显著下降。这些结果首次证明了沙眼衣原体（L2血清型）与淋巴细胞的结合，代表了在体外将细菌与淋巴细胞之间的物理相互作用与特定免疫刺激活性相关联的直接步骤。
