Bard J, Hall J, Levitt D
Guthrie Research Institute of the Guthrie Foundation for Medical Research, Sayre, PA 18840.
J Gen Microbiol. 1987 Apr;133(4):899-910. doi: 10.1099/00221287-133-4-899.
As a model system for analysing interactions between chlamydiae and myeloid cells and their precursors, we have studied binding, ingestion and destruction of Chlamydia trachomatis (L2 serovar) by the human promyelocytic cell line HL-60. HL-60 cells were induced by phorbol myristate acetate (PMA) and dimethyl sulphoxide (DMSO) to differentiate along either the macrophage or the granulocyte pathway, respectively. Using an immunofluorescence assay and electron microscopy, we have shown that induced (differentiated) HL-60 cells, but not uninduced (undifferentiated) HL-60 or other cell lines treated with PMA or DMSO, exhibit increased binding, ingestion and elimination of C. trachomatis; these activities are associated with specific histochemical and antigenic markers of myeloid differentiation. These results suggest that myeloid cells acquire the ability to interact with and kill chlamydiae during cell development.
作为分析衣原体与髓样细胞及其前体之间相互作用的模型系统，我们研究了人早幼粒细胞系HL-60对沙眼衣原体（L2血清型）的结合、摄取和破坏情况。HL-60细胞分别用佛波酯肉豆蔻酸乙酸酯（PMA）和二甲基亚砜（DMSO）诱导，沿巨噬细胞或粒细胞途径分化。通过免疫荧光测定和电子显微镜，我们发现诱导（分化）的HL-60细胞，而非未诱导（未分化）的HL-60细胞或用PMA或DMSO处理的其他细胞系，对沙眼衣原体的结合、摄取和清除增加；这些活性与髓样分化的特定组织化学和抗原标志物相关。这些结果表明，髓样细胞在细胞发育过程中获得了与衣原体相互作用并杀死衣原体的能力。
