Liu Chunru, Radebe Stoffel Matjeke, Zhang Hang, Jia Junpeng, Xie Shuang, Shi Miaolei, Yu Qinghua
MOE Joint International Research Laboratory of Animal Health and Food Safety, College of Veterinary Medicine, Nanjing Agricultural University, Weigang 1, Nanjing, Jiangsu, 210095, PR China.
MOE Joint International Research Laboratory of Animal Health and Food Safety, College of Veterinary Medicine, Nanjing Agricultural University, Weigang 1, Nanjing, Jiangsu, 210095, PR China.
Vet Microbiol. 2022 Mar;266:109357. doi: 10.1016/j.vetmic.2022.109357. Epub 2022 Jan 25.
Bacillus coagulans (B. coagulans), a spore-forming bacteria, has been further studied for its high tolerance to extreme environmental stressors and probiotic characteristics. But the modulatory effect of B. coagulans on the intestinal mucosal barrier remains unclear. To investigate the effects of B. coagulans on intestinal mucosal barrier, 1-day-old broiler chickens were orally administrated with 10 CFU/mL B. coagulans for consecutive 42 days. In this study, the body weight, jejunum villus height and crypt depth of broiler chickens were significantly increased after B. coagulans treatment. B. coagulans also increased the contents of total protein (TP) and albumin (ALB) in serum, and reduced the contents of low-density lipoprotein (LDL-C), blood urea nitrogen (BUN) and triglyceride (TG). In addition, B. coagulans improved the intestinal flora, significantly increasing the relative abundance of beneficial bacteria in the intestine. The ability of B. coagulans to enhance innate immunity is observed by the increased number of goblet cells and the decreased mRNA expression of IL-1β, IL-6, TNF-α, and sIgA content. Moreover, B. coagulans promoted intestinal epithelial proliferation through the Wnt/β-catenin signaling pathway. This study demonstrated that B. coagulans could maintain the intestinal mucosal barrier by improving the intestinal flora, enhancing innate immunity and promoting intestinal epithelial proliferation.
凝结芽孢杆菌是一种形成芽孢的细菌，因其对极端环境应激源具有高耐受性和益生菌特性而得到进一步研究。但凝结芽孢杆菌对肠道黏膜屏障的调节作用仍不清楚。为了研究凝结芽孢杆菌对肠道黏膜屏障的影响，对1日龄肉鸡连续42天口服给予10 CFU/mL的凝结芽孢杆菌。在本研究中，凝结芽孢杆菌处理后肉鸡的体重、空肠绒毛高度和隐窝深度显著增加。凝结芽孢杆菌还增加了血清中总蛋白（TP）和白蛋白（ALB）的含量，并降低了低密度脂蛋白（LDL-C）、血尿素氮（BUN）和甘油三酯（TG）的含量。此外，凝结芽孢杆菌改善了肠道菌群，显著增加了肠道中有益菌的相对丰度。通过杯状细胞数量增加以及白细胞介素-1β（IL-1β）、白细胞介素-6（IL-6）、肿瘤坏死因子-α（TNF-α）的mRNA表达降低和分泌型免疫球蛋白A（sIgA）含量降低，观察到凝结芽孢杆菌增强先天免疫的能力。此外，凝结芽孢杆菌通过Wnt/β-连环蛋白信号通路促进肠道上皮细胞增殖。本研究表明，凝结芽孢杆菌可通过改善肠道菌群、增强先天免疫和促进肠道上皮细胞增殖来维持肠道黏膜屏障。
