葡萄糖对老年大鼠离体胰岛胰岛素释放及离子运动的影响。
The effect of glucose on insulin release and ion movements in isolated pancreatic islets of rats in old age.
作者信息
Ammon H P, Fahmy A, Mark M, Wahl M A, Youssif N
机构信息
Department of Pharmacology, University of Tuebingen, F.R.G.
出版信息
J Physiol. 1987 Mar;384:347-54. doi: 10.1113/jphysiol.1987.sp016458.
Abstract
- The effect of glucose on 86Rb+ efflux, 45Ca2+ net uptake and insulin secretion of pancreatic islets from 3- and 24-month-old rats was studied. 2. Raising the glucose concentration from 3 to 5.6 and 16.7 mM had no effect on 86Rb+ efflux from islets of 24-month-old male rats whereas that from 24-month-old female rats was decreased. 3. At 16.7 mM-glucose, net uptake of 45Ca2+ was significantly diminished in islets of 24-month-old rats compared to islets of 3-month-old rats. 4. In the presence of 16.7 mM-glucose, islets of 24-month-old rats exhibited only 60-70% of the insulin release obtained with islets from 3-month-old rats. 5. Neither net uptake of 45Ca2+ nor insulin secretion appear to differ between the sexes. 6. These data suggest that the decreased insulin secretory response to glucose during old age is due, at least in part, to inadequate inhibition of K+ efflux and diminished net uptake of Ca2+.
摘要
- 研究了葡萄糖对3月龄和24月龄大鼠胰岛86Rb+外流、45Ca2+净摄取及胰岛素分泌的影响。2. 将葡萄糖浓度从3 mM提高到5.6 mM和16.7 mM，对24月龄雄性大鼠胰岛的86Rb+外流没有影响，而24月龄雌性大鼠胰岛的86Rb+外流则减少。3. 在16.7 mM葡萄糖条件下，与3月龄大鼠胰岛相比，24月龄大鼠胰岛的45Ca2+净摄取显著减少。4. 在16.7 mM葡萄糖存在的情况下，24月龄大鼠胰岛的胰岛素释放量仅为3月龄大鼠胰岛的60 - 70%。5. 45Ca2+净摄取和胰岛素分泌在性别之间似乎没有差异。6. 这些数据表明，老年期对葡萄糖的胰岛素分泌反应降低至少部分是由于对K+外流的抑制不足和Ca2+净摄取减少所致。
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