State Key Laboratory of Analytical Chemistry for Life Science and Collaborative Innovation Center of Chemistry for Life Sciences, School of Chemistry and Chemical Engineering, Nanjing University , Nanjing 210023, China.
Anal Chem. 2017 Aug 1;89(15):8050-8056. doi: 10.1021/acs.analchem.7b01494. Epub 2017 Jul 10.
We report a multicolor ECL device based on closed bipolar electrode (BPE) for the visualized sensing of prostate-specific antigen (PSA) in human blood serum. As the emission color of concomitant electrochemiluminophores is potential resolved, similar to a three-electrode system, selective excitation of ECL could be achieved by tuning the interfacial potential at the poles of BPE. Via modulating the resistance of BPE, multicolor ECL emission of [Ru(bpy)] and [Ir(ppy)] mixture using tripropylamine (TPrA) as the co-reactant was observed at the anode and the principle was elaborated. The concept was utilized to the quantification of clinical biomarkers with the color variation. A PSA concentration dependent silver bridge was constructed in the gap of the BPEs as an electric conductivity modulator. On the basis of a multicolor BPE-ECL device, the cutoff values (4.0 and 10.0 ng/mL) of human PSA could be recognized with naked eyes by the green-yellow-red ECL emission changing. As the first multicolor ECL device in biological analysis, BPE may raise the application of potential-resolved ECL to a new level.
我们报告了一种基于闭合双极电极 (BPE) 的多色 ECL 器件，用于可视化检测人血清中的前列腺特异性抗原 (PSA)。由于伴随的电化学发光体的发射颜色可以潜在地分辨，类似于三电极系统，可以通过调节 BPE 两极的界面电势来实现 ECL 的选择性激发。通过调节 BPE 的电阻，使用三丙胺 (TPrA) 作为共反应物，在阳极观察到 [Ru(bpy)] 和 [Ir(ppy)] 混合物的多色 ECL 发射，并阐述了其原理。该概念被用于通过颜色变化定量检测临床生物标志物。在 BPE 的间隙中构建了一个 PSA 浓度依赖性的银桥作为电导率调节剂。基于多色 BPE-ECL 器件，通过绿色-黄色-红色 ECL 发射的变化，可以用肉眼识别出 PSA 的截断值 (4.0 和 10.0 ng/mL)。作为生物分析中的第一个多色 ECL 器件，BPE 可以将潜在分辨 ECL 的应用提升到一个新的水平。
