State Key Laboratory of Analytical Chemistry for Life Science and Collaborative Innovation Center of Chemistry for Life Sciences, School of Chemistry and Chemical Engineering , Nanjing University , Nanjing 210023 , P. R. China.
Anal Chem. 2018 Mar 6;90(5):3570-3575. doi: 10.1021/acs.analchem.8b00014. Epub 2018 Feb 16.
A selective excitation of [Ir(df-ppy)(pic)] and [Ru(bpy)] through tuning the electrode potential is reported in this work. Bidirectional color change from blue-green to red could be observed along with increase and decrease of the potential, which was ascribed to the dual-potential excitation property of [Ir(df-ppy)(pic)]. Similar to the three-electrode system, selective excitation of ECL could be achieved at the anode of the bipolar electrode (BPE). Both increase and decrease of the faradic reactions at the cathode of the BPE could induce ECL reporting color at the other pole switched from blue-green to red. We applied a closed BPE device for the bioanalysis of multicolor ECL since the organic solvent containing electrochemiluminophores could be separated from the bioanalytes. On the basis of BPE arrays coupled with the ECL switch, the detection of three biomarkers of prostate cancer, PSA, microRNA-141, and sarcosine were integrated in a same device. The cutoff values of the biomarkers could be recognized directly by the naked eye. Such a device holds great potential in the early diagnosis of prostate cancer.
本文报道了通过调节电极电位选择性激发[Ir(df-ppy)(pic)]和[Ru(bpy)]。随着电位的增加和减少，可以观察到从蓝绿色到红色的双向颜色变化，这归因于[Ir(df-ppy)(pic)]的双电位激发特性。与三电极系统类似，在双极电极（BPE）的阳极也可以实现 ECL 的选择性激发。BPE 阴极的法拉第反应的增加和减少都可以诱导在另一极的 ECL 报告颜色从蓝绿色变为红色。我们应用了一个封闭的 BPE 设备进行多色 ECL 的生物分析，因为含有电化学发光体的有机溶剂可以与生物分析物分离。基于 BPE 阵列与 ECL 开关的结合，我们将前列腺癌的三种生物标志物 PSA、microRNA-141 和肌氨酸的检测集成在同一个设备中。通过肉眼可以直接识别生物标志物的截止值。这种设备在前列腺癌的早期诊断中具有很大的潜力。
