使用基因编码荧光生物传感器监测生物体发育过程中的NAD(H)和NADP(H)动态变化。
Monitoring NAD(H) and NADP(H) dynamics during organismal development with genetically encoded fluorescent biosensors.
作者信息
Li Ting, Zou Yejun, Liu Shuning, Yang Yi, Zhang Zhuo, Zhao Yuzheng
机构信息
Optogenetics & Synthetic Biology Interdisciplinary Research Center, State Key Laboratory of Bioreactor Engineering, Shanghai Frontiers Science Center of Optogenetic Techniques for Cell Metabolism, East China University of Science and Technology, 130 Mei Long Road, Shanghai, 200237, China.
Shanghai Key Laboratory of New Drug Design, School of Pharmacy, East China University of Science and Technology, 130 Mei Long Road, Shanghai, 200237, China.
出版信息
Cell Regen. 2022 Feb 1;11(1):5. doi: 10.1186/s13619-021-00105-4.
Cell metabolism plays vital roles in organismal development, but it has been much less studied than transcriptional and epigenetic control of developmental programs. The difficulty might be largely attributed to the lack of in situ metabolite assays. Genetically encoded fluorescent sensors are powerful tools for noninvasive metabolic monitoring in living cells and in vivo by highly spatiotemporal visualization. Among all living organisms, the NAD(H) and NADP(H) pools are essential for maintaining redox homeostasis and for modulating cellular metabolism. Here, we introduce NAD(H) and NADP(H) biosensors, present example assays in developing organisms, and describe promising prospects for how sensors contribute to developmental biology research.
细胞代谢在机体发育中起着至关重要的作用，但与发育程序的转录和表观遗传控制相比，对其研究要少得多。这种困难可能很大程度上归因于缺乏原位代谢物检测方法。基因编码荧光传感器是通过高度时空可视化在活细胞和体内进行无创代谢监测的强大工具。在所有生物中，NAD(H)和NADP(H)库对于维持氧化还原稳态和调节细胞代谢至关重要。在这里，我们介绍NAD(H)和NADP(H)生物传感器，展示在发育生物体中的示例检测方法，并描述传感器对发育生物学研究贡献的广阔前景。
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