Optogenetics & Synthetic Biology Interdisciplinary Research Center, Shanghai Frontiers Science Center of Optogenetic Techniques for Cell Metabolism, State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, 130 Mei Long Road, Shanghai 200237, China; Shanghai Key Laboratory of New Drug Design, School of Pharmacy, East China University of Science and Technology, 130 Mei Long Road, Shanghai 200237, China.
Optogenetics & Synthetic Biology Interdisciplinary Research Center, Shanghai Frontiers Science Center of Optogenetic Techniques for Cell Metabolism, State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, 130 Mei Long Road, Shanghai 200237, China.
Mech Ageing Dev. 2022 Jun;204:111657. doi: 10.1016/j.mad.2022.111657. Epub 2022 Mar 12.
Nicotinamide adenine dinucleotide (NAD) plays vital roles in cell metabolism, cell signaling, and gene expression regulation. NAD shows intricate subcellular distribution and dynamic changes in diverse biological processes; however, traditional biochemical assays usually require cell lysis, making it technically difficult to measure NAD metabolism in live cells or in vivo. Recently, a few genetically encoded and semisynthetic fluorescent sensors have been used to monitor NAD metabolism in a variety of biological settings. In this review, we summarize the recent progress in the development of fluorescent sensors for NAD and their applications in life science research. We further analyze their advantages, limitations, and perspectives for future development.
烟酰胺腺嘌呤二核苷酸 (NAD) 在细胞代谢、细胞信号转导和基因表达调控中发挥着至关重要的作用。NAD 在各种生物过程中表现出复杂的亚细胞分布和动态变化；然而，传统的生化分析通常需要细胞裂解，因此在活细胞或体内测量 NAD 代谢在技术上具有一定难度。最近，一些遗传编码和半合成荧光传感器已被用于监测各种生物环境中的 NAD 代谢。在这篇综述中，我们总结了 NAD 荧光传感器的最新研究进展及其在生命科学研究中的应用。我们进一步分析了它们的优缺点以及未来发展的前景。
