Mazier D, Mellouk S, Beaudoin R L, Texier B, Druilhe P, Hockmeyer W, Trosper J, Paul C, Charoenvit Y, Young J
Science. 1986 Jan 10;231(4734):156-9. doi: 10.1126/science.3510455.
Antibodies were raised in mice immunized with several recombinant and synthetic peptides of the circumsporozoite protein of Plasmodium falciparum. The antibodies were evaluated for protective activity in a human hepatocyte culture system. They exerted their protective effect against the parasite at three points: sporozoite attachment to the hepatocyte surface, entry, and subsequent intracellular development. Inhibition of attachment and entry were found to be related to the antibody titer against the authentic circumsporozoite protein on the sporozoite surface, especially when peptides were administered with alum or complete Freund's adjuvant. Even when invasion was not totally inhibited, the presence of abnormal trophozoites and a frequent inhibition of schizont development in long-term cultures suggested continued activity of antibodies at the intracellular level after sporozoite penetration had been completed.
用恶性疟原虫环子孢子蛋白的几种重组肽和合成肽免疫小鼠，产生了抗体。在人肝细胞培养系统中评估了这些抗体的保护活性。它们在三个方面对寄生虫发挥保护作用：子孢子附着于肝细胞表面、进入细胞以及随后的细胞内发育。发现附着和进入的抑制与针对子孢子表面真实环子孢子蛋白的抗体效价有关，尤其是当肽与明矾或完全弗氏佐剂一起给药时。即使入侵没有被完全抑制，长期培养中异常滋养体的存在以及裂殖体发育的频繁抑制表明，在子孢子穿透完成后，抗体在细胞内水平仍持续发挥作用。
