Wirtz R A, Ballou W R, Schneider I, Chedid L, Gross M J, Young J F, Hollingdale M, Diggs C L, Hockmeyer W T
Exp Parasitol. 1987 Apr;63(2):166-72. doi: 10.1016/0014-4894(87)90158-5.
The immunogenicity of Plasmodium falciparum recombinant circumsporozoite protein constructs R16tet32, R32tet32, and R48tet32 in mice was examined by measuring antibody responses by enzyme linked immunosorbent assay, immunofluorescence, circumsporozoite precipitation, and inhibition of sporozoite invasion. All three constructs were found to be immunogenic when administered alone, but antibody responses were greater for the larger constructs, R32tet32 and R48tet32. Increased dose, boosting, and the use of adjuvants further augmented antibody responses. R32tet32 was found to be the most immunogenic of the three constructs, and high levels of protective antibodies were found to persist for at least 44 weeks when the construct was given with alum. Clinical trials with alum adjuvanted R32tet32 have now begun.
通过酶联免疫吸附测定、免疫荧光、环子孢子沉淀和抑制子孢子入侵来测量抗体反应，从而检测恶性疟原虫重组环子孢子蛋白构建体R16tet32、R32tet32和R48tet32在小鼠中的免疫原性。发现所有这三种构建体单独给药时均具有免疫原性，但较大的构建体R32tet32和R48tet32的抗体反应更强。增加剂量、加强免疫以及使用佐剂可进一步增强抗体反应。发现R32tet32是这三种构建体中免疫原性最强的，当该构建体与明矾一起给予时，高水平的保护性抗体至少持续44周。目前已开始对明矾佐剂R32tet32进行临床试验。
