Fine J D, Gay S
Arch Dermatol. 1986 Jan;122(1):48-51. doi: 10.1001/archderm.122.1.48.
To determine the diagnostic usefulness of a monoclonal antibody to the LDA-1 antigen, a newly defined noncollagenous component of the lamina densa of human basement membrane, we examined skin specimens from 20 patients with epidermolysis bullosa (simplex type, eight patients; junctional type, four patients; dystrophic type, eight patients) by the indirect immunofluorescence technique. We compared this antibody to polyclonal antibodies with three other antigens routinely examined in diagnostic immunofluorescence mapping studies. The antigen defined by the LDA-1 monoclonal antibody was expressed normally in all specimens examined, regardless of the type of epidermolysis bullosa present. Furthermore, no disparity was noted between the LDA-1 monoclonal antibody and a polyclonal antibody to type IV collagen in the microscopic localization of binding within induced skin blisters. These findings suggest that the LDA-1 monoclonal antibody is an excellent immunoreagent for diagnostic immunofluorescence mapping studies.
为了确定一种针对LDA-1抗原（人基底膜致密层新定义的非胶原蛋白成分）的单克隆抗体的诊断效用，我们采用间接免疫荧光技术检查了20例大疱性表皮松解症患者（单纯型8例、交界型4例、营养不良型8例）的皮肤标本。我们将该抗体与在诊断性免疫荧光图谱研究中常规检测的其他三种抗原的多克隆抗体进行了比较。无论存在何种类型的大疱性表皮松解症，LDA-1单克隆抗体所定义的抗原在所有检查的标本中均正常表达。此外，在诱导皮肤水疱内的结合微观定位方面，LDA-1单克隆抗体与IV型胶原多克隆抗体之间未发现差异。这些发现表明，LDA-1单克隆抗体是诊断性免疫荧光图谱研究的一种优秀免疫试剂。
